An orthotopic skull base model of malignant meningioma

Gilson S. Baia, Eduard B. Dinca, Tomoko Ozawa, Edna T. Kimura, Michael W. McDermott, C. David James, Scott R. VandenBerg, Anita Lal

Research output: Contribution to journalArticle

Abstract

Meningioma tumor growth involves the subarachnoid space that contains the cerebrospinal fluid. Modeling tumor growth in this microenvironment has been associated with widespread leptomeningeal dissemination, which is uncharacteristic of human meningiomas. Consequently, survival times and tumor properties are varied, limiting their utility in testing experimental therapies. We report the development and characterization of a reproducible orthotopic skull-base meningioma model in athymic mice using the IOMM-Lee cell line. Localized tumor growth was obtained by using optimal cell densities and matrigel as the implantation medium. Survival times were within a narrow range of 17-21 days. The xenografts grew locally compressing surrounding brain tissue. These tumors had histopathologic characteristics of anaplastic meningiomas including high cellularity, nuclear pleomorphism, cellular pattern loss, necrosis and conspicuous mitosis. Similar to human meningiomas, considerable invasion of the dura and skull and some invasion of adjacent brain along perivascular tracts were observed. The pattern of hypoxia was also similar to human malignant meningiomas. We use bioluminescent imaging to non-invasively monitor the growth of the xenografts and determine the survival benefit from temozolomide treatment. Thus, we describe a malignant meningioma model system that will be useful for investigating the biology of meningiomas and for preclinical assessment of therapeutic agents.

Original languageEnglish (US)
Pages (from-to)172-179
Number of pages8
JournalBrain Pathology
Volume18
Issue number2
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Skull Base
Meningioma
temozolomide
Neoplasms
Growth
Heterografts
Survival
Subarachnoid Space
Investigational Therapies
Brain
Mitosis
Skull
Nude Mice
Cerebrospinal Fluid
Necrosis
Cell Count
Cell Line
Therapeutics

Keywords

  • Bioluminescent imaging
  • IOMM-Lee
  • Meningioma
  • Orthotopic
  • Skull base
  • Xenografts

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine

Cite this

Baia, G. S., Dinca, E. B., Ozawa, T., Kimura, E. T., McDermott, M. W., James, C. D., ... Lal, A. (2008). An orthotopic skull base model of malignant meningioma. Brain Pathology, 18(2), 172-179. https://doi.org/10.1111/j.1750-3639.2007.00109.x

An orthotopic skull base model of malignant meningioma. / Baia, Gilson S.; Dinca, Eduard B.; Ozawa, Tomoko; Kimura, Edna T.; McDermott, Michael W.; James, C. David; VandenBerg, Scott R.; Lal, Anita.

In: Brain Pathology, Vol. 18, No. 2, 04.2008, p. 172-179.

Research output: Contribution to journalArticle

Baia, GS, Dinca, EB, Ozawa, T, Kimura, ET, McDermott, MW, James, CD, VandenBerg, SR & Lal, A 2008, 'An orthotopic skull base model of malignant meningioma', Brain Pathology, vol. 18, no. 2, pp. 172-179. https://doi.org/10.1111/j.1750-3639.2007.00109.x
Baia GS, Dinca EB, Ozawa T, Kimura ET, McDermott MW, James CD et al. An orthotopic skull base model of malignant meningioma. Brain Pathology. 2008 Apr;18(2):172-179. https://doi.org/10.1111/j.1750-3639.2007.00109.x
Baia, Gilson S. ; Dinca, Eduard B. ; Ozawa, Tomoko ; Kimura, Edna T. ; McDermott, Michael W. ; James, C. David ; VandenBerg, Scott R. ; Lal, Anita. / An orthotopic skull base model of malignant meningioma. In: Brain Pathology. 2008 ; Vol. 18, No. 2. pp. 172-179.
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