An orthopedic tissue adhesive for targeted delivery of intraoperative biologics

Jacob Simson, Joshua Crist, Iossif Strehin, Qiaozhi Lu, Jennifer H. Elisseeff

Research output: Contribution to journalArticle

Abstract

Tissue adhesives can bind together damaged tissues and serve as tools to deliver and localize therapeutics to facilitate regeneration. One emerging therapeutic trend in orthopedics is the use of intraoperative biologics (IOB), such as bone marrow (BM) and platelet-rich plasma (PRP), to stimulate healing. Here, we introduce the application of the biomaterial chondroitin sulfate succinimidyl succinate (CS-NHS) to deliver IOB in a hydrogel adhesive. We demonstrate the biomaterial's ability to bind various tissue types and its cellular biocompatibility with encapsulated human mesenchymal stem cells (hMSCs). Further, we examine in detail the CS-NHS adhesive combined with BM aspirate for use in bone applications. hMSCs were encapsulated in CS-BM and cultured for 5 weeks in osteogenic medium. Quantitative RT-PCR demonstrated osteogenesis via upregulation of the osteogenic transcription factor Runx2 and bone markers alkaline phosphatase and osteocalcin. Significant deposition of calcium and osteocalcin was detected using biochemical, histological, and immunohistochemical techniques. Shear testing demonstrated that the CS-BM adhesive exhibited an adhesive strength approximately an order of magnitude stronger than fibrin glue and approaching that of a cyanoacrylate adhesive. These results indicate that CS-NHS is a promising delivery tool for IOB in orthopedic applications requiring a strong, degradable, and biocompatible adhesive that supports bone growth.

Original languageEnglish (US)
Pages (from-to)392-400
Number of pages9
JournalJournal of Orthopaedic Research
Volume31
Issue number3
DOIs
StatePublished - Mar 1 2013

Keywords

  • PRP
  • bone marrow
  • intraoperative biologics
  • osteogenesis
  • tissue adhesive

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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