An Orchestrated Program Regulating Secretory Pathway Genes and Cargos by the Transmembrane Transcription Factor CREB-H

Sónia Barbosa, Giovanna Fasanella, Suzanne Carreira, Marta Llarena, Rebecca Fox, Cristina Barreca, Deborah J Andrew, Peter O'Hare

Research output: Contribution to journalArticle

Abstract

CREB3 proteins comprise a set of ER-localized bZip transcription factors defined by the presence of a transmembrane domain. They are regulated by inter-compartmental transport, Golgi cleavage and nuclear transport where they promote appropriate transcriptional responses. Although CREB3 proteins play key roles in differentiation, inflammation and metabolism, a general framework relating their defining features to these diverse activities is lacking. We identify unique features of CREB3 organization including the ATB domain, which we show it is essential for transcriptional activity. This domain is absent in all other human bZip factors, but conserved in Drosophila CREBA, which controls secretory pathway genes (SPGs). Furthermore, each of the five human CREB3 factors was capable of activating SPGs in Drosophila, dependent upon the ATB domain. Expression of the CREB3 protein, CREB-H, in 293 cells, upregulated genes involved in secretory capacity, extracellular matrix formation and lipid metabolism and increased secretion of specific cargos. In liver cells, which normally express CREB-H, the active form specifically induced secretion of apolipoproteins, including ApoA-IV, ApoAI, consistent with data implicating CREB-H in metabolic homeostasis. Based on these data and other recent studies, we propose a general role for the CREB3 family in regulating secretory capacity, with particular relevance to specialized cargos.

Original languageEnglish (US)
Pages (from-to)382-398
Number of pages17
JournalTraffic
Volume14
Issue number4
DOIs
StatePublished - Apr 2013

Fingerprint

Secretory Pathway
Transcription Factors
Genes
Human engineering
Drosophila
Apolipoproteins A
Cyclic AMP Response Element-Binding Protein
Cell Nucleus Active Transport
Apolipoproteins
Lipid Metabolism
Metabolism
Liver
Extracellular Matrix
Proteins
Homeostasis
Inflammation

Keywords

  • ApoA-IV
  • Apolipoproteins
  • CREB-H
  • CREB3L3
  • CREBA
  • Extracellular matrix
  • Lipid metabolism
  • Secretion

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Barbosa, S., Fasanella, G., Carreira, S., Llarena, M., Fox, R., Barreca, C., ... O'Hare, P. (2013). An Orchestrated Program Regulating Secretory Pathway Genes and Cargos by the Transmembrane Transcription Factor CREB-H. Traffic, 14(4), 382-398. https://doi.org/10.1111/tra.12038

An Orchestrated Program Regulating Secretory Pathway Genes and Cargos by the Transmembrane Transcription Factor CREB-H. / Barbosa, Sónia; Fasanella, Giovanna; Carreira, Suzanne; Llarena, Marta; Fox, Rebecca; Barreca, Cristina; Andrew, Deborah J; O'Hare, Peter.

In: Traffic, Vol. 14, No. 4, 04.2013, p. 382-398.

Research output: Contribution to journalArticle

Barbosa, S, Fasanella, G, Carreira, S, Llarena, M, Fox, R, Barreca, C, Andrew, DJ & O'Hare, P 2013, 'An Orchestrated Program Regulating Secretory Pathway Genes and Cargos by the Transmembrane Transcription Factor CREB-H', Traffic, vol. 14, no. 4, pp. 382-398. https://doi.org/10.1111/tra.12038
Barbosa, Sónia ; Fasanella, Giovanna ; Carreira, Suzanne ; Llarena, Marta ; Fox, Rebecca ; Barreca, Cristina ; Andrew, Deborah J ; O'Hare, Peter. / An Orchestrated Program Regulating Secretory Pathway Genes and Cargos by the Transmembrane Transcription Factor CREB-H. In: Traffic. 2013 ; Vol. 14, No. 4. pp. 382-398.
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