An optimized set of human telomere clones for studying telomere integrity and architecture

Samantha J.L. Knigh, Christa M. Lese, Kathrin S. Precht, Julie Kuc, Yi Ning, Sarah Lucas, Regina Regan, Mary Brenan, Alison Nicod, N. Martin Lawrei, Donald L.N. Cardy, Huy Nguyen, Thomas J. Hudson, Harold C. Riethman, David H. Ledbetter, Jonathan Flint

Research output: Contribution to journalArticlepeer-review

Abstract

Telomere-specific clones are a valuable resource for the characterization of chromosomal rearrangements. We previously reported a first-generation set of human telomere probes consisting of 34 genomic clones, which were a known distance from the end of the chromosome (˜300 kb), and 7 clones corresponding to the most distal markers on the integrated genetic/physical map (1p, 5p, 6p, 9p, 12p, 15q, and 20q). Subsequently, this resource has been optimized and completed: the size of the genomic clones has been expanded to a target size of 100-200 kb, which is optimal for use in genome-scanning methodologies, and additional probes for the remaining seven telomeres have been identified. For each clone we give an associated mapped sequence-tagged site and provide distances from the telomere estimated using a combination of fiberFISH, interphase FISH, sequence analysis, and radiation-hybrid mapping. This updated set of telomeric clones is an invaluable resource for clinical diagnosis and represents an important contribution to genetic and physical mapping efforts aimed at telomeric regions.

Original languageEnglish (US)
Article number62644
Pages (from-to)320-332
Number of pages13
JournalAmerican journal of human genetics
Volume67
Issue number2
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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