An integrated genetic linkage map of the laboratory rat

Donna M. Brown, Tara C. Matise, George Koike, Jason S. Simon, Eric S. Winer, Sarah Zangen, Michael G. McLaughlin, Masahide Shiozawa, O. Scott Atkinson, James R. Hudson, Aravinda Chakravarti, Eric S. Lander, Howard J. Jacob

Research output: Contribution to journalArticle

Abstract

The laboratory rat, Rattus novegicus, is a major model system for physiological and pathophysiological studies, and since 1966 more than 422,000 publications describe biological studies on the rat (NCBI/Medline). The rat is becoming an increasingly important genetic model for the study of specific diseases, as well as retaining its role as a major preclinical model system for pharmaceutical development. The initial genetic linkage map of the rat contained 432 genetic markers (Jacob et al. 1995) out of 1171 developed due to the relatively low polymorphism rate of the mapping cross used (SHR x BN) when compared to the interspecific crosses in the mouse. While the rat genome project continues to localize additional markers on the linkage map, and as of 11/97 more than 3,200 loci have been mapped. Current map construction is using two different crosses (SHRSP x BN and FHH x ACI) rather than the initial mapping cross. Consequently there is a need to provide integration among the different maps. We set out to develop an integrated map, as well as increase the number of markers on the rat genetic map. The crosses available for this analysis included the original mapping cross SHR x BN reciprocal F2 intercross (448 markers), a GH x BN intercross (205 markers), a SS/Mcw x BN intercross (235 markers), and a FHH/Eur x ACI/Hsd intercross (276 markers), which is also one of the new mapping crosses. Forty-six animals from each cross were genotyped with markers polymorphic for that cross. The maps appear to cover the vast majority of the rat genome. The availability of these additional markers should facilitate more complete whole genome scans in a greater number of strains and provide additional markers in specific genomic regions of interest.

Original languageEnglish (US)
Pages (from-to)521-530
Number of pages10
JournalMammalian Genome
Volume9
Issue number7
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Genetic Linkage
Genome
Genetic Models
Genetic Markers
Publications

ASJC Scopus subject areas

  • Genetics

Cite this

Brown, D. M., Matise, T. C., Koike, G., Simon, J. S., Winer, E. S., Zangen, S., ... Jacob, H. J. (1998). An integrated genetic linkage map of the laboratory rat. Mammalian Genome, 9(7), 521-530. https://doi.org/10.1007/s003359900812

An integrated genetic linkage map of the laboratory rat. / Brown, Donna M.; Matise, Tara C.; Koike, George; Simon, Jason S.; Winer, Eric S.; Zangen, Sarah; McLaughlin, Michael G.; Shiozawa, Masahide; Atkinson, O. Scott; Hudson, James R.; Chakravarti, Aravinda; Lander, Eric S.; Jacob, Howard J.

In: Mammalian Genome, Vol. 9, No. 7, 1998, p. 521-530.

Research output: Contribution to journalArticle

Brown, DM, Matise, TC, Koike, G, Simon, JS, Winer, ES, Zangen, S, McLaughlin, MG, Shiozawa, M, Atkinson, OS, Hudson, JR, Chakravarti, A, Lander, ES & Jacob, HJ 1998, 'An integrated genetic linkage map of the laboratory rat', Mammalian Genome, vol. 9, no. 7, pp. 521-530. https://doi.org/10.1007/s003359900812
Brown DM, Matise TC, Koike G, Simon JS, Winer ES, Zangen S et al. An integrated genetic linkage map of the laboratory rat. Mammalian Genome. 1998;9(7):521-530. https://doi.org/10.1007/s003359900812
Brown, Donna M. ; Matise, Tara C. ; Koike, George ; Simon, Jason S. ; Winer, Eric S. ; Zangen, Sarah ; McLaughlin, Michael G. ; Shiozawa, Masahide ; Atkinson, O. Scott ; Hudson, James R. ; Chakravarti, Aravinda ; Lander, Eric S. ; Jacob, Howard J. / An integrated genetic linkage map of the laboratory rat. In: Mammalian Genome. 1998 ; Vol. 9, No. 7. pp. 521-530.
@article{a0ed2a1a9c5a4677bca38ad0cb8f4efe,
title = "An integrated genetic linkage map of the laboratory rat",
abstract = "The laboratory rat, Rattus novegicus, is a major model system for physiological and pathophysiological studies, and since 1966 more than 422,000 publications describe biological studies on the rat (NCBI/Medline). The rat is becoming an increasingly important genetic model for the study of specific diseases, as well as retaining its role as a major preclinical model system for pharmaceutical development. The initial genetic linkage map of the rat contained 432 genetic markers (Jacob et al. 1995) out of 1171 developed due to the relatively low polymorphism rate of the mapping cross used (SHR x BN) when compared to the interspecific crosses in the mouse. While the rat genome project continues to localize additional markers on the linkage map, and as of 11/97 more than 3,200 loci have been mapped. Current map construction is using two different crosses (SHRSP x BN and FHH x ACI) rather than the initial mapping cross. Consequently there is a need to provide integration among the different maps. We set out to develop an integrated map, as well as increase the number of markers on the rat genetic map. The crosses available for this analysis included the original mapping cross SHR x BN reciprocal F2 intercross (448 markers), a GH x BN intercross (205 markers), a SS/Mcw x BN intercross (235 markers), and a FHH/Eur x ACI/Hsd intercross (276 markers), which is also one of the new mapping crosses. Forty-six animals from each cross were genotyped with markers polymorphic for that cross. The maps appear to cover the vast majority of the rat genome. The availability of these additional markers should facilitate more complete whole genome scans in a greater number of strains and provide additional markers in specific genomic regions of interest.",
author = "Brown, {Donna M.} and Matise, {Tara C.} and George Koike and Simon, {Jason S.} and Winer, {Eric S.} and Sarah Zangen and McLaughlin, {Michael G.} and Masahide Shiozawa and Atkinson, {O. Scott} and Hudson, {James R.} and Aravinda Chakravarti and Lander, {Eric S.} and Jacob, {Howard J.}",
year = "1998",
doi = "10.1007/s003359900812",
language = "English (US)",
volume = "9",
pages = "521--530",
journal = "Mammalian Genome",
issn = "0938-8990",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - An integrated genetic linkage map of the laboratory rat

AU - Brown, Donna M.

AU - Matise, Tara C.

AU - Koike, George

AU - Simon, Jason S.

AU - Winer, Eric S.

AU - Zangen, Sarah

AU - McLaughlin, Michael G.

AU - Shiozawa, Masahide

AU - Atkinson, O. Scott

AU - Hudson, James R.

AU - Chakravarti, Aravinda

AU - Lander, Eric S.

AU - Jacob, Howard J.

PY - 1998

Y1 - 1998

N2 - The laboratory rat, Rattus novegicus, is a major model system for physiological and pathophysiological studies, and since 1966 more than 422,000 publications describe biological studies on the rat (NCBI/Medline). The rat is becoming an increasingly important genetic model for the study of specific diseases, as well as retaining its role as a major preclinical model system for pharmaceutical development. The initial genetic linkage map of the rat contained 432 genetic markers (Jacob et al. 1995) out of 1171 developed due to the relatively low polymorphism rate of the mapping cross used (SHR x BN) when compared to the interspecific crosses in the mouse. While the rat genome project continues to localize additional markers on the linkage map, and as of 11/97 more than 3,200 loci have been mapped. Current map construction is using two different crosses (SHRSP x BN and FHH x ACI) rather than the initial mapping cross. Consequently there is a need to provide integration among the different maps. We set out to develop an integrated map, as well as increase the number of markers on the rat genetic map. The crosses available for this analysis included the original mapping cross SHR x BN reciprocal F2 intercross (448 markers), a GH x BN intercross (205 markers), a SS/Mcw x BN intercross (235 markers), and a FHH/Eur x ACI/Hsd intercross (276 markers), which is also one of the new mapping crosses. Forty-six animals from each cross were genotyped with markers polymorphic for that cross. The maps appear to cover the vast majority of the rat genome. The availability of these additional markers should facilitate more complete whole genome scans in a greater number of strains and provide additional markers in specific genomic regions of interest.

AB - The laboratory rat, Rattus novegicus, is a major model system for physiological and pathophysiological studies, and since 1966 more than 422,000 publications describe biological studies on the rat (NCBI/Medline). The rat is becoming an increasingly important genetic model for the study of specific diseases, as well as retaining its role as a major preclinical model system for pharmaceutical development. The initial genetic linkage map of the rat contained 432 genetic markers (Jacob et al. 1995) out of 1171 developed due to the relatively low polymorphism rate of the mapping cross used (SHR x BN) when compared to the interspecific crosses in the mouse. While the rat genome project continues to localize additional markers on the linkage map, and as of 11/97 more than 3,200 loci have been mapped. Current map construction is using two different crosses (SHRSP x BN and FHH x ACI) rather than the initial mapping cross. Consequently there is a need to provide integration among the different maps. We set out to develop an integrated map, as well as increase the number of markers on the rat genetic map. The crosses available for this analysis included the original mapping cross SHR x BN reciprocal F2 intercross (448 markers), a GH x BN intercross (205 markers), a SS/Mcw x BN intercross (235 markers), and a FHH/Eur x ACI/Hsd intercross (276 markers), which is also one of the new mapping crosses. Forty-six animals from each cross were genotyped with markers polymorphic for that cross. The maps appear to cover the vast majority of the rat genome. The availability of these additional markers should facilitate more complete whole genome scans in a greater number of strains and provide additional markers in specific genomic regions of interest.

UR - http://www.scopus.com/inward/record.url?scp=15444351630&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15444351630&partnerID=8YFLogxK

U2 - 10.1007/s003359900812

DO - 10.1007/s003359900812

M3 - Article

C2 - 9657848

AN - SCOPUS:15444351630

VL - 9

SP - 521

EP - 530

JO - Mammalian Genome

JF - Mammalian Genome

SN - 0938-8990

IS - 7

ER -