An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in Russia

Yanina Balabanova, Francis Drobniewski, Vladyslav Nikolayevskyy, Annika Kruuner, Nadezhda Malomanova, Tatyana Simak, Nailya Ilyina, Svetlana Zakharova, Natalya Lebedeva, Heather L. Alexander, Rick O'Brien, Hojoon Sohn, Anastasia Shakhmistova, Ivan Fedorin

Research output: Contribution to journalArticle

Abstract

Objective: To analyse the feasibility, cost and performance of rapid tuberculosis (TB) molecular and culture systems, in a high multidrug-resistant TB (MDR TB) middle-income region (Samara, Russia) and provide evidence for WHO policy change. Methods: Performance and cost evaluation was conducted to compare the BACTEC™ MGIT™ 960 system for culture and drug susceptibility testing (DST) and molecular systems for TB diagnosis, resistance to isoniazid and rifampin, and MDR TB identification compared to conventional Lowenstein-Jensen culture assays. Findings: 698 consecutive patients (2487 sputum samples) with risk factors for drug-resistant tuberculosis were recruited. Overall M. tuberculosis complex culture positivity rates were 31.6% (787/2487) in MGIT and 27.1% (675/2487) in LJ (90.5% and 83.2% for smear-positive specimens). In total, 809 cultures of M. tuberculosis complex were isolated by any method. Median time to detection was 14 days for MGIT and 36 days for LJ (10 and 33 days for smear positive specimens) and indirect DST in MGIT took 9 days compared to 21 days on LJ. There was good concordance between DST on LJ and MGIT (96.8% for rifampin and 95.6% for isoniazid). Both molecular hybridization assay results correlated well with MGIT DST results, although molecular assays generally yielded higher rates of resistance (by approximately 3% for both isoniazid and rifampin). Conclusion: With effective planning and logistics, the MGIT 960 and molecular based methodologies can be successfully introduced into a reference laboratory setting in a middle incidence country. High rates of MDR TB in the Russian Federation make the introduction of such assays particularly useful.

Original languageEnglish (US)
Article numbere7129
JournalPloS one
Volume4
Issue number9
DOIs
StatePublished - Sep 23 2009
Externally publishedYes

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multiple drug resistance
Russia
Multiple Drug Resistance
tuberculosis
Isoniazid
Tuberculosis
Rifampin
isoniazid
Assays
Multidrug-Resistant Tuberculosis
rifampicin
liquids
Liquids
drugs
Pharmaceutical Preparations
Testing
assays
Costs and Cost Analysis
Sputum
methodology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Balabanova, Y., Drobniewski, F., Nikolayevskyy, V., Kruuner, A., Malomanova, N., Simak, T., ... Fedorin, I. (2009). An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in Russia. PloS one, 4(9), [e7129]. https://doi.org/10.1371/journal.pone.0007129

An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in Russia. / Balabanova, Yanina; Drobniewski, Francis; Nikolayevskyy, Vladyslav; Kruuner, Annika; Malomanova, Nadezhda; Simak, Tatyana; Ilyina, Nailya; Zakharova, Svetlana; Lebedeva, Natalya; Alexander, Heather L.; O'Brien, Rick; Sohn, Hojoon; Shakhmistova, Anastasia; Fedorin, Ivan.

In: PloS one, Vol. 4, No. 9, e7129, 23.09.2009.

Research output: Contribution to journalArticle

Balabanova, Y, Drobniewski, F, Nikolayevskyy, V, Kruuner, A, Malomanova, N, Simak, T, Ilyina, N, Zakharova, S, Lebedeva, N, Alexander, HL, O'Brien, R, Sohn, H, Shakhmistova, A & Fedorin, I 2009, 'An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in Russia', PloS one, vol. 4, no. 9, e7129. https://doi.org/10.1371/journal.pone.0007129
Balabanova, Yanina ; Drobniewski, Francis ; Nikolayevskyy, Vladyslav ; Kruuner, Annika ; Malomanova, Nadezhda ; Simak, Tatyana ; Ilyina, Nailya ; Zakharova, Svetlana ; Lebedeva, Natalya ; Alexander, Heather L. ; O'Brien, Rick ; Sohn, Hojoon ; Shakhmistova, Anastasia ; Fedorin, Ivan. / An integrated approach to rapid diagnosis of tuberculosis and multidrug resistance using liquid culture and molecular methods in Russia. In: PloS one. 2009 ; Vol. 4, No. 9.
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AU - Malomanova, Nadezhda

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