An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease

Teresa C.F. Assumpção; Ivo M.B. Francischetti; John F. Andersen; Alexandra Schwarz; Jaime M. Santana; José M.C. Ribeiro

doi:10.1016/j.ibmb.2007.11.001

An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease

Teresa C.F. Assumpção, Ivo M.B. Francischetti, John F. Andersen, Alexandra Schwarz, Jaime M. Santana, José M.C. Ribeiro

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

Triatoma infestans is a hemiptera, vector of Chagas' disease that feeds exclusively on vertebrate blood in all life stages. Hematophagous insects' salivary glands (SG) produce potent pharmacological compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cDNA library from its SG was randomly sequenced. Also, salivary proteins were submitted to two-dimensional gel (2D-gel) electrophoresis followed by MS analysis. We present the analysis of a set of 1534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results.

Original language	English (US)
Pages (from-to)	213-232
Number of pages	20
Journal	Insect Biochemistry and Molecular Biology
Volume	38
Issue number	2
DOIs	https://doi.org/10.1016/j.ibmb.2007.11.001
State	Published - Feb 2008
Externally published	Yes

Keywords

Feeding
Hematophagy
Saliva
Salivary gland
Sialome
Transcriptome
Triatoma infestans

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

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10.1016/j.ibmb.2007.11.001

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title = "An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease",

abstract = "Triatoma infestans is a hemiptera, vector of Chagas' disease that feeds exclusively on vertebrate blood in all life stages. Hematophagous insects' salivary glands (SG) produce potent pharmacological compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cDNA library from its SG was randomly sequenced. Also, salivary proteins were submitted to two-dimensional gel (2D-gel) electrophoresis followed by MS analysis. We present the analysis of a set of 1534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results.",

keywords = "Feeding, Hematophagy, Saliva, Salivary gland, Sialome, Transcriptome, Triatoma infestans",

author = "Assump{\c c}{\~a}o, {Teresa C.F.} and Francischetti, {Ivo M.B.} and Andersen, {John F.} and Alexandra Schwarz and Santana, {Jaime M.} and Ribeiro, {Jos{\'e} M.C.}",

note = "Funding Information: This work was supported in part by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases. TCFA was a recipient of a CAPES (Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior, Ministry of Education, Brazil) fellowship. We are grateful to the NIAID Research Technologies Branch, directed by Dr. Robert Hohman, for support in the proteomic studies, to Nancy Shulman for editorial assistance to Chuong Huynh from NCBI for help with posting the data, and Drs. Robert Gwadz and Katherine Zoon for encouragement and support. ",

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AU - Andersen, John F.

AU - Schwarz, Alexandra

AU - Santana, Jaime M.

AU - Ribeiro, José M.C.

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KW - Transcriptome

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An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease

Abstract

Keywords

ASJC Scopus subject areas

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