An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease

Teresa C.F. Assumpção, Ivo M.B. Francischetti, John F. Andersen, Alexandra Schwarz, Jaime M. Santana, José M.C. Ribeiro

Research output: Contribution to journalArticlepeer-review

Abstract

Triatoma infestans is a hemiptera, vector of Chagas' disease that feeds exclusively on vertebrate blood in all life stages. Hematophagous insects' salivary glands (SG) produce potent pharmacological compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cDNA library from its SG was randomly sequenced. Also, salivary proteins were submitted to two-dimensional gel (2D-gel) electrophoresis followed by MS analysis. We present the analysis of a set of 1534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results.

Original languageEnglish (US)
Pages (from-to)213-232
Number of pages20
JournalInsect Biochemistry and Molecular Biology
Volume38
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Keywords

  • Feeding
  • Hematophagy
  • Saliva
  • Salivary gland
  • Sialome
  • Transcriptome
  • Triatoma infestans

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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