TY - JOUR
T1 - An insight into the sialome of the adult female mosquito Aedes albopictus
AU - Arcà, Bruno
AU - Lombardo, Fabrizio
AU - Francischetti, Ivo M.B.
AU - Pham, Van My
AU - Mestres-Simon, Montserrat
AU - Andersen, John F.
AU - Ribeiro, José M.C.
N1 - Funding Information:
This work was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health (J.M.C.R.) and in part by grants from the European Union (M. Coluzzi and BA; BioMalPar N 503578). We thank Mark Garfield from the NIAID Research Technologies Branch for performing the Edman degration reactions, the NIAID intramural editor Brenda Rae Marshall for assistance, and Chuong Huynh (NCBI) for help with posting the data. We also thank Roberto Romi for providing Ae. albopictus eggs and Maria Calzetta for technical assistance.
PY - 2007/2
Y1 - 2007/2
N2 - To gain insight into the molecular repertoire of the adult female salivary glands of the tiger mosquito Aedes albopictus, we performed transcriptome and proteome analysis. cDNA clones were sequenced and assembled in clusters of related sequences and the corresponding genes assigned to one of three categories: housekeeping (H; 31%), secreted (S; 34%), or unknown (U; 35%) function. Among the putative secreted factors are proteins known to be widely distributed in the saliva of blood-sucking Diptera, such as D7 and antigen 5 family members, as well as proteins that are mosquito- or culicine-specific, i.e., the 30-kDa allergen or the 62-kDa and 34-kDa families, respectively. Expression of 15 of these salivary proteins was confirmed by Edman degradation. Tissue and sex specificity of selected transcripts were evaluated by RT-PCR and identified at least 32 genes whose expression is restricted or enriched in the female salivary glands of Ae. albopictus, whereas 17 additional genes were expressed in female glands and adult males but not in other tissues of adult females. For approximately one third of the genes analyzed, involvement in blood-feeding, sugar digestion, immune response, or other more generic physiological roles can be postulated; however, no functions can be suggested for the remaining sequences, which therefore likely represent either novel functions or novel molecules recruited during the evolution of hematophagy. Supplemental spreadsheets with hyperlinks to all sequences used in this manuscript are hyperlinked throughout the text and can be found at http://www.ncbi.nlm.nih.gov/projects/omes/#salivarytranscriptomes.
AB - To gain insight into the molecular repertoire of the adult female salivary glands of the tiger mosquito Aedes albopictus, we performed transcriptome and proteome analysis. cDNA clones were sequenced and assembled in clusters of related sequences and the corresponding genes assigned to one of three categories: housekeeping (H; 31%), secreted (S; 34%), or unknown (U; 35%) function. Among the putative secreted factors are proteins known to be widely distributed in the saliva of blood-sucking Diptera, such as D7 and antigen 5 family members, as well as proteins that are mosquito- or culicine-specific, i.e., the 30-kDa allergen or the 62-kDa and 34-kDa families, respectively. Expression of 15 of these salivary proteins was confirmed by Edman degradation. Tissue and sex specificity of selected transcripts were evaluated by RT-PCR and identified at least 32 genes whose expression is restricted or enriched in the female salivary glands of Ae. albopictus, whereas 17 additional genes were expressed in female glands and adult males but not in other tissues of adult females. For approximately one third of the genes analyzed, involvement in blood-feeding, sugar digestion, immune response, or other more generic physiological roles can be postulated; however, no functions can be suggested for the remaining sequences, which therefore likely represent either novel functions or novel molecules recruited during the evolution of hematophagy. Supplemental spreadsheets with hyperlinks to all sequences used in this manuscript are hyperlinked throughout the text and can be found at http://www.ncbi.nlm.nih.gov/projects/omes/#salivarytranscriptomes.
KW - Aedes albopictus
KW - Hematophagy
KW - Mosquito
KW - Saliva
KW - Salivary proteins
KW - Transcriptome
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U2 - 10.1016/j.ibmb.2006.10.007
DO - 10.1016/j.ibmb.2006.10.007
M3 - Article
C2 - 17244540
AN - SCOPUS:33846235966
SN - 0965-1748
VL - 37
SP - 107
EP - 127
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 2
ER -