An inducible nitric-oxide synthase (NOS)-associated protein inhibits NOS dimerization and activity

Edward A. Ratovitski, Clare Bao, Richard A. Quick, Audrey McMillan, Chaim Kozlovsky, Charles J. Lowenstein

Research output: Contribution to journalArticle

Abstract

A variety of transcriptional and post-transcriptional mechanisms regulate the expression of the inducible nitric-oxide synthase (iNOS, or NOS2). Although neurons and endothelial cells express proteins that interact with and inhibit neuronal NOS and endothelial NOS, macrophage proteins that inhibit NOS2 have not been identified. We show that murine macrophages express a 110-kDa protein that interacts with NOS2, which we call NOS- associated protein-110 kDa (NAP110). NAP110 directly interacts with the amino terminus of NOS2, and inhibits NOS catalytic activity by preventing formation of NOS2 homodimers. Expression of NAP110 may be a mechanism by which macrophages expressing NOS2 protect themselves from cytotoxic levels of nitric oxide.

Original languageEnglish (US)
Pages (from-to)30250-30257
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number42
DOIs
StatePublished - Oct 15 1999

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Dimerization
Nitric Oxide Synthase Type II
Nitric Oxide Synthase
Macrophages
Proteins
Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
Endothelial cells
Neurons
Catalyst activity
Nitric Oxide
Endothelial Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ratovitski, E. A., Bao, C., Quick, R. A., McMillan, A., Kozlovsky, C., & Lowenstein, C. J. (1999). An inducible nitric-oxide synthase (NOS)-associated protein inhibits NOS dimerization and activity. Journal of Biological Chemistry, 274(42), 30250-30257. https://doi.org/10.1074/jbc.274.42.30250

An inducible nitric-oxide synthase (NOS)-associated protein inhibits NOS dimerization and activity. / Ratovitski, Edward A.; Bao, Clare; Quick, Richard A.; McMillan, Audrey; Kozlovsky, Chaim; Lowenstein, Charles J.

In: Journal of Biological Chemistry, Vol. 274, No. 42, 15.10.1999, p. 30250-30257.

Research output: Contribution to journalArticle

Ratovitski, EA, Bao, C, Quick, RA, McMillan, A, Kozlovsky, C & Lowenstein, CJ 1999, 'An inducible nitric-oxide synthase (NOS)-associated protein inhibits NOS dimerization and activity', Journal of Biological Chemistry, vol. 274, no. 42, pp. 30250-30257. https://doi.org/10.1074/jbc.274.42.30250
Ratovitski, Edward A. ; Bao, Clare ; Quick, Richard A. ; McMillan, Audrey ; Kozlovsky, Chaim ; Lowenstein, Charles J. / An inducible nitric-oxide synthase (NOS)-associated protein inhibits NOS dimerization and activity. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 42. pp. 30250-30257.
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