An in vitro natural degeneration model of chondrocytes derived from end-plate of intervertebral discs of rats

Ji Xiang Shi, Yong Jun Wang, Qi Shi, Quan Zhou, Peng Sun, Qin Bian, Chong Jian Zhou

Research output: Contribution to journalArticle

Abstract

Objective: To set up a natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats in order to offer an appropriate carrier for the study on mechanism of intervertebral disc degeneration. Methods: The method of enzyme digestion combined with natural subculture was used to set up the in vitro natural degeneration model of chondrocytes derived from the endplate of intervertebral disc of rats. The morphological appearances and microstructures of the chondrocytes of different generations were observed. The expression of collagen II in chondrocytes was detected by immunocytochemical method. Results: The chondrocytes derived from the endplate of intervertebral disc expressed collagen II. After 13 days of culture, the chondrocytes of generation III showed that the ability of cell division descended, the nucleoli became unclear, the cells deformed obviously, fusiform shape with weak optical activity appeared, and the intercellular space was enlarged. There were vacuoles and lipid droplets in cytoplasm. The synthesis of collagen II, as well as the cell proliferation rate, descended notably. All results showed the natural degeneration process of the chondrocytes. Conclusion: The in vitro natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats was successfully established. This can offer the cytological basis for study on the mechanism of intervertebral disc degeneration.

Original languageEnglish (US)
Pages (from-to)293-297
Number of pages5
JournalJournal of Chinese Integrative Medicine
Volume4
Issue number3
DOIs
StatePublished - May 2006
Externally publishedYes

Keywords

  • Animal models
  • Chondrocyte of the endplate
  • Degeneration
  • Intervertebral disc

ASJC Scopus subject areas

  • Complementary and alternative medicine

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