@article{3a6b49927e89461bb98ef128c119a512,
title = "An improved surface passivation method for single-molecule studies",
abstract = "We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.",
author = "Boyang Hua and Han, {Kyu Young} and Ruobo Zhou and Hajin Kim and Xinghua Shi and Abeysirigunawardena, {Sanjaya C.} and Ankur Jain and Digvijay Singh and Vasudha Aggarwal and Woodson, {Sarah A.} and Taekjip Ha",
note = "Funding Information: laboratory at Georgia Institute of Technology and H.R. Koh of the S. Myong laboratory at the University of Illinois for performing independent tests of our protocols; and W. Cheng (University of Michigan) for providing the Alexa 594–labeled protein G B1 domain sample. This work was supported by a US National Science Foundation grant (PHY 1430124 to T.H.) and US National Institutes of Health grants (GM065367 and AG042332 to T.H. and GM60819 to S.A.W.). T.H. is also supported by the Howard Hughes Medical Institute.",
year = "2014",
month = nov,
day = "25",
doi = "10.1038/nmeth.3143",
language = "English (US)",
volume = "11",
pages = "1233--1236",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "12",
}