TY - JOUR
T1 - An improved simple method of mouse lung intubation
AU - MacDonald, Kelvin D.
AU - Chang, Herng Yu Sucie
AU - Mitzner, Wayne
PY - 2009/3
Y1 - 2009/3
N2 - Given the ubiquitous use of mice to study lung disease, it is curious that more investigators do not use repeated intubation to study mechanical and cellular changes in individual mice. One of the reasons for this limited use of intubation is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper, we describe a complete procedure, including novel approaches that simplify this intubation, so that it can be routinely accomplished with relatively little training. The technique can also be set up with relatively little expense and expertise. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice and potentially increasing the statistical power by using each mouse as its own control.
AB - Given the ubiquitous use of mice to study lung disease, it is curious that more investigators do not use repeated intubation to study mechanical and cellular changes in individual mice. One of the reasons for this limited use of intubation is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper, we describe a complete procedure, including novel approaches that simplify this intubation, so that it can be routinely accomplished with relatively little training. The technique can also be set up with relatively little expense and expertise. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice and potentially increasing the statistical power by using each mouse as its own control.
KW - Broncoalveolar lavage
KW - Longitudinal studies
KW - Lung mechanics
KW - Trachea
UR - http://www.scopus.com/inward/record.url?scp=64349114243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64349114243&partnerID=8YFLogxK
U2 - 10.1152/japplphysiol.91376.2008
DO - 10.1152/japplphysiol.91376.2008
M3 - Article
C2 - 19150857
AN - SCOPUS:64349114243
SN - 8750-7587
VL - 106
SP - 984
EP - 987
JO - Journal of applied physiology
JF - Journal of applied physiology
IS - 3
ER -