An improved methodology for routine in vivo microdialysis in non-human primates

Bhaskar S. Kolachana, Richard C. Saunders, Daniel R. Weinberger

Research output: Contribution to journalArticlepeer-review

Abstract

A new method was developed to carry out in vivo microdialysis experiments repetitively and routinely within an individual monkey. We designed and built a dialysis probe guide and holding device ('guide holder') which permits accurate placement of dialysis probes into cortical (e.g., prefrontal, hippocampus, and parietal cortices) and subcortical target areas (e.g., caudate nucleus, amygdala, and nucleus accumbens) of the rhesus monkey brain without extensive and repetitive surgery needed to expose a desired brain region. The guide holder is positioned, using MRI-guided coordinates, and fixed to the skull over an intended targeted region. This design provides an opportunity to conduct several experiments in a single monkey over an extended period and permits placement of several probes accurately into 'fresh' or 'experienced' tissue during repeated microdialysis experiments. In addition, during repeated dialysis experiments tissue trauma is minimized because no surgical procedure is necessary on the day of dialysate collection. This procedure can be readily adapted for use with an awake monkey.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Neuroscience Methods
Volume55
Issue number1
DOIs
StatePublished - Nov 1994
Externally publishedYes

Keywords

  • Dialysis
  • Dopamine
  • Guide holder
  • Magnetic resonance imaging
  • Stereotaxy

ASJC Scopus subject areas

  • Neuroscience(all)

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