TY - JOUR
T1 - An improved method for generating and identifying human induced pluripotent stem cells.
AU - Mali, Prashant
AU - Ye, Zhaohui
AU - Chou, Bin Kuan
AU - Yen, Jonathan
AU - Cheng, Linzhao
PY - 2010
Y1 - 2010
N2 - This chapter describes the methods we use to derive and characterize human induced pluripotent stem (iPS) cells. We describe in order, first our culture techniques for the starting fibroblast populations and methods for retrovirus preparation and concentration. Subsequently, a detailed iPS derivation protocol suitable for human fibroblast populations is discussed using standard retroviral vectors expressing the classic four or three reprogramming genes. Finally, we elaborate a robust technique for monitoring and identification of potential iPS cells through live staining of reprogrammed cells. We also outline steps for characterization of the resulting iPS cell lines.
AB - This chapter describes the methods we use to derive and characterize human induced pluripotent stem (iPS) cells. We describe in order, first our culture techniques for the starting fibroblast populations and methods for retrovirus preparation and concentration. Subsequently, a detailed iPS derivation protocol suitable for human fibroblast populations is discussed using standard retroviral vectors expressing the classic four or three reprogramming genes. Finally, we elaborate a robust technique for monitoring and identification of potential iPS cells through live staining of reprogrammed cells. We also outline steps for characterization of the resulting iPS cell lines.
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U2 - 10.1007/978-1-60761-691-7_12
DO - 10.1007/978-1-60761-691-7_12
M3 - Article
C2 - 20336524
AN - SCOPUS:77953095271
SN - 1064-3745
VL - 636
SP - 191
EP - 205
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -