An immunosuppressed Syrian golden hamster model for SARS-CoV infection

Scott R. Schaecher, Jennifer Stabenow, Christina Oberle, Jill Schriewer, R. Mark Buller, John E. Sagartz, Andrew Pekosz

Research output: Contribution to journalArticle

Abstract

Several small animal models have been developed for the study of severe acute respiratory syndrome coronavirus (SARS-CoV) replication and pathogenesis. Syrian golden hamsters are among the best small animal models, though little clinical illness and no mortality are observed after virus infection. Cyclophosphamide was used to immunosuppress hamsters leading to a prolonged disease course and higher mortality after SARS-CoV infection. In addition, there was a significant weight loss, expanded tissue tropism, and increased viral pathology in the lung, heart, kidney, and nasal turbinate tissues. Infection with recombinant SARS-CoV viruses bearing disruptions in the gene 7 coding region showed no significant change in replication kinetics, tissue tropism, morbidity, or mortality suggesting that the ORF7a (7a) and ORF7b (7b) proteins are not required for virus replication in immunosuppressed hamsters. This modified hamster model may provide a useful tool for SARS-CoV pathogenesis studies, evaluation of antiviral therapy, and analysis of additional SARS-CoV mutants.

Original languageEnglish (US)
Pages (from-to)312-321
Number of pages10
JournalVirology
Volume380
Issue number2
DOIs
StatePublished - Oct 25 2008

Keywords

  • Accessory gene
  • Coronavirus
  • Cyclophosphamide
  • Hamster
  • ORF7a
  • ORF7b
  • Pathogenesis
  • SARS-CoV

ASJC Scopus subject areas

  • Virology

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  • Cite this

    Schaecher, S. R., Stabenow, J., Oberle, C., Schriewer, J., Buller, R. M., Sagartz, J. E., & Pekosz, A. (2008). An immunosuppressed Syrian golden hamster model for SARS-CoV infection. Virology, 380(2), 312-321. https://doi.org/10.1016/j.virol.2008.07.026