An immunoradiometric assay for the quantification of Plasmodium sporozoite invasion of HepG2 cells

Research output: Contribution to journalArticle

Abstract

Malaria infection in a vertebrate host is initiated when Plasmodium sporozoites invade hepatocytes after injection by an infected mosquito. In vitro, the parasites invade and develop in HepG2 cells and these cells have been used to study target cell invasion by sporozoites. Previously described in vitro invasion assays involve staining and counting of intracellular sporozoites or exoerythrocytic forms of the parasite. Here we describe an immunoradiometric assay that can quantify sporozoite invasion of HepG2 cells in vitro. The assay relies on the differential detection of intracellular and extracellular circumsporozoite protein (CS; the major surface protein of the sporozoite) which can then be used to calculate the efficiency of invasion. Since this assay can be performed more rapidly than the current assays in which parasites must be counted under a microscope, it enables investigators to more rapidly screen inhibitors of sporozoite invasion.

Original languageEnglish (US)
Pages (from-to)17-23
Number of pages7
JournalJournal of Immunological Methods
Volume221
Issue number1-2
DOIs
StatePublished - Dec 1998
Externally publishedYes

Fingerprint

Immunoradiometric Assay
Sporozoites
Plasmodium
Hep G2 Cells
Parasites
Culicidae
Malaria
Vertebrates
Hepatocytes
Membrane Proteins
Research Personnel
Staining and Labeling
Injections
Infection
In Vitro Techniques
Proteins

Keywords

  • HepG2
  • Immunoradiometric
  • Plasmodium
  • Quantification
  • Sporozoite

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

An immunoradiometric assay for the quantification of Plasmodium sporozoite invasion of HepG2 cells. / Sinnis, Photini.

In: Journal of Immunological Methods, Vol. 221, No. 1-2, 12.1998, p. 17-23.

Research output: Contribution to journalArticle

@article{9b4e6c21b0a848c3997c351841ca5342,
title = "An immunoradiometric assay for the quantification of Plasmodium sporozoite invasion of HepG2 cells",
abstract = "Malaria infection in a vertebrate host is initiated when Plasmodium sporozoites invade hepatocytes after injection by an infected mosquito. In vitro, the parasites invade and develop in HepG2 cells and these cells have been used to study target cell invasion by sporozoites. Previously described in vitro invasion assays involve staining and counting of intracellular sporozoites or exoerythrocytic forms of the parasite. Here we describe an immunoradiometric assay that can quantify sporozoite invasion of HepG2 cells in vitro. The assay relies on the differential detection of intracellular and extracellular circumsporozoite protein (CS; the major surface protein of the sporozoite) which can then be used to calculate the efficiency of invasion. Since this assay can be performed more rapidly than the current assays in which parasites must be counted under a microscope, it enables investigators to more rapidly screen inhibitors of sporozoite invasion.",
keywords = "HepG2, Immunoradiometric, Plasmodium, Quantification, Sporozoite",
author = "Photini Sinnis",
year = "1998",
month = "12",
doi = "10.1016/S0022-1759(98)00151-3",
language = "English (US)",
volume = "221",
pages = "17--23",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - An immunoradiometric assay for the quantification of Plasmodium sporozoite invasion of HepG2 cells

AU - Sinnis, Photini

PY - 1998/12

Y1 - 1998/12

N2 - Malaria infection in a vertebrate host is initiated when Plasmodium sporozoites invade hepatocytes after injection by an infected mosquito. In vitro, the parasites invade and develop in HepG2 cells and these cells have been used to study target cell invasion by sporozoites. Previously described in vitro invasion assays involve staining and counting of intracellular sporozoites or exoerythrocytic forms of the parasite. Here we describe an immunoradiometric assay that can quantify sporozoite invasion of HepG2 cells in vitro. The assay relies on the differential detection of intracellular and extracellular circumsporozoite protein (CS; the major surface protein of the sporozoite) which can then be used to calculate the efficiency of invasion. Since this assay can be performed more rapidly than the current assays in which parasites must be counted under a microscope, it enables investigators to more rapidly screen inhibitors of sporozoite invasion.

AB - Malaria infection in a vertebrate host is initiated when Plasmodium sporozoites invade hepatocytes after injection by an infected mosquito. In vitro, the parasites invade and develop in HepG2 cells and these cells have been used to study target cell invasion by sporozoites. Previously described in vitro invasion assays involve staining and counting of intracellular sporozoites or exoerythrocytic forms of the parasite. Here we describe an immunoradiometric assay that can quantify sporozoite invasion of HepG2 cells in vitro. The assay relies on the differential detection of intracellular and extracellular circumsporozoite protein (CS; the major surface protein of the sporozoite) which can then be used to calculate the efficiency of invasion. Since this assay can be performed more rapidly than the current assays in which parasites must be counted under a microscope, it enables investigators to more rapidly screen inhibitors of sporozoite invasion.

KW - HepG2

KW - Immunoradiometric

KW - Plasmodium

KW - Quantification

KW - Sporozoite

UR - http://www.scopus.com/inward/record.url?scp=0032433140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032433140&partnerID=8YFLogxK

U2 - 10.1016/S0022-1759(98)00151-3

DO - 10.1016/S0022-1759(98)00151-3

M3 - Article

C2 - 9894894

AN - SCOPUS:0032433140

VL - 221

SP - 17

EP - 23

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-2

ER -