An immunohistochemical study of the innervation of the ureterovesical junction in infancy and childhood

I. S. Dixon, D. A. Canning, John Phillip Gearhart, J. A. Gosling

Research output: Contribution to journalArticle

Abstract

Objective To use histological and immunohistochemical methods to study the structure and innervation of the human ureterovesical junction (UVJ). Materials and methods A series of 24 post-natal specimens taken from patients ranging in age from 1 month to 6 years were examined. Routine histological slides were stained with Masson's trichrome. In addition, an indirect immunohistochemical method was used to study the occurrence and distribution of nerves immunoreactive for the neuropeptides vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), substance P (SP) and calcitonin gene-related peptide (CGRP). Immunoreactivity to tyrosine hydroxylase (TH), dopamine-B-hydroxylase (DBH) and to protein gene product (PGP) 9.5, a general nerve marker, were also studied. Results The UVJ comprised a ureteric muscle component (the intramural ureter) and a detrusor component (the immediately adjacent region of the urinary bladder). In the majority of specimens a third or intermediate layer was also present. This additional component consisted of tightly-packed smooth muscle cells which formed an incomplete layer that partially surrounded the juxtavesical and intramural parts of the ureter. Numerous PGP-, VIP-, NPY, DBH- and TH- like immunoreactive (-LIR) nerves were associated with the smooth muscle bundles which comprised the intramural ureter. Such nerves ran in the connective tissue separating ureteric smooth muscle bundles and rarely coursed amongst individual smooth muscle cells comprising each bundle. SP-and CGRP-containing nerves were rarely observed in association with the intramural ureter and none were detected in the ureteric submucosa. The intermediate muscle layer was richly innervated by PGP-, TH-, DBH- and NPY- containing nerves which ran amongst the smooth muscle cells comprising this layer. VIP-, SP- and CGRP-LIR nerves were not observed within the intermediate layer. The detrusor component of the UVJ was innervated by PGP-, NPY- and VIP-LIR nerves which frequently extended between the smooth muscle cells forming the detrusor muscle bundles. TH-, DBH-, SP- and CGRP- LIR nerve fibres were rarely encountered. Conclusion These findings indicate that noradrenergic nerves play a major role in the control of the ureteric component of the UVJ. In addition, the present results form baseline morphological data with which to compare the results of future studies on the structure of the UVJ in cases of vesicoureteric reflux.

Original languageEnglish (US)
Pages (from-to)292-297
Number of pages6
JournalBritish Journal of Urology
Volume73
Issue number3
StatePublished - 1994

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Calcitonin Gene-Related Peptide
Neuropeptide Y
Vasoactive Intestinal Peptide
Tyrosine 3-Monooxygenase
Ureter
Substance P
Mixed Function Oxygenases
Smooth Muscle Myocytes
Dopamine
Muscles
Smooth Muscle
Proteins
Neuropeptides
Nerve Fibers
Connective Tissue
Urinary Bladder

Keywords

  • human
  • Immunohistochemistry
  • innervation
  • post-natal ureterovesical junction

ASJC Scopus subject areas

  • Urology

Cite this

An immunohistochemical study of the innervation of the ureterovesical junction in infancy and childhood. / Dixon, I. S.; Canning, D. A.; Gearhart, John Phillip; Gosling, J. A.

In: British Journal of Urology, Vol. 73, No. 3, 1994, p. 292-297.

Research output: Contribution to journalArticle

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abstract = "Objective To use histological and immunohistochemical methods to study the structure and innervation of the human ureterovesical junction (UVJ). Materials and methods A series of 24 post-natal specimens taken from patients ranging in age from 1 month to 6 years were examined. Routine histological slides were stained with Masson's trichrome. In addition, an indirect immunohistochemical method was used to study the occurrence and distribution of nerves immunoreactive for the neuropeptides vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), substance P (SP) and calcitonin gene-related peptide (CGRP). Immunoreactivity to tyrosine hydroxylase (TH), dopamine-B-hydroxylase (DBH) and to protein gene product (PGP) 9.5, a general nerve marker, were also studied. Results The UVJ comprised a ureteric muscle component (the intramural ureter) and a detrusor component (the immediately adjacent region of the urinary bladder). In the majority of specimens a third or intermediate layer was also present. This additional component consisted of tightly-packed smooth muscle cells which formed an incomplete layer that partially surrounded the juxtavesical and intramural parts of the ureter. Numerous PGP-, VIP-, NPY, DBH- and TH- like immunoreactive (-LIR) nerves were associated with the smooth muscle bundles which comprised the intramural ureter. Such nerves ran in the connective tissue separating ureteric smooth muscle bundles and rarely coursed amongst individual smooth muscle cells comprising each bundle. SP-and CGRP-containing nerves were rarely observed in association with the intramural ureter and none were detected in the ureteric submucosa. The intermediate muscle layer was richly innervated by PGP-, TH-, DBH- and NPY- containing nerves which ran amongst the smooth muscle cells comprising this layer. VIP-, SP- and CGRP-LIR nerves were not observed within the intermediate layer. The detrusor component of the UVJ was innervated by PGP-, NPY- and VIP-LIR nerves which frequently extended between the smooth muscle cells forming the detrusor muscle bundles. TH-, DBH-, SP- and CGRP- LIR nerve fibres were rarely encountered. Conclusion These findings indicate that noradrenergic nerves play a major role in the control of the ureteric component of the UVJ. In addition, the present results form baseline morphological data with which to compare the results of future studies on the structure of the UVJ in cases of vesicoureteric reflux.",
keywords = "human, Immunohistochemistry, innervation, post-natal ureterovesical junction",
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AU - Canning, D. A.

AU - Gearhart, John Phillip

AU - Gosling, J. A.

PY - 1994

Y1 - 1994

N2 - Objective To use histological and immunohistochemical methods to study the structure and innervation of the human ureterovesical junction (UVJ). Materials and methods A series of 24 post-natal specimens taken from patients ranging in age from 1 month to 6 years were examined. Routine histological slides were stained with Masson's trichrome. In addition, an indirect immunohistochemical method was used to study the occurrence and distribution of nerves immunoreactive for the neuropeptides vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), substance P (SP) and calcitonin gene-related peptide (CGRP). Immunoreactivity to tyrosine hydroxylase (TH), dopamine-B-hydroxylase (DBH) and to protein gene product (PGP) 9.5, a general nerve marker, were also studied. Results The UVJ comprised a ureteric muscle component (the intramural ureter) and a detrusor component (the immediately adjacent region of the urinary bladder). In the majority of specimens a third or intermediate layer was also present. This additional component consisted of tightly-packed smooth muscle cells which formed an incomplete layer that partially surrounded the juxtavesical and intramural parts of the ureter. Numerous PGP-, VIP-, NPY, DBH- and TH- like immunoreactive (-LIR) nerves were associated with the smooth muscle bundles which comprised the intramural ureter. Such nerves ran in the connective tissue separating ureteric smooth muscle bundles and rarely coursed amongst individual smooth muscle cells comprising each bundle. SP-and CGRP-containing nerves were rarely observed in association with the intramural ureter and none were detected in the ureteric submucosa. The intermediate muscle layer was richly innervated by PGP-, TH-, DBH- and NPY- containing nerves which ran amongst the smooth muscle cells comprising this layer. VIP-, SP- and CGRP-LIR nerves were not observed within the intermediate layer. The detrusor component of the UVJ was innervated by PGP-, NPY- and VIP-LIR nerves which frequently extended between the smooth muscle cells forming the detrusor muscle bundles. TH-, DBH-, SP- and CGRP- LIR nerve fibres were rarely encountered. Conclusion These findings indicate that noradrenergic nerves play a major role in the control of the ureteric component of the UVJ. In addition, the present results form baseline morphological data with which to compare the results of future studies on the structure of the UVJ in cases of vesicoureteric reflux.

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