An immunoglobulin E-dependent recombinant histamine-releasing factor induces interleukin-4 secretion from human basophils

John Thomas Schroeder, Lawrence M. Lichtenstein, Susan M MacDonald

Research output: Contribution to journalArticle

Abstract

A novel recombinant histamine-releasing factor (rHFR), which stimulates secretion from a subpopulation of human basophils that express a particular type of immunoglobulin E (IgE) or IgE+, was found to induce interleukin-4 (IL-4) production from cells isolated from these same donors. The secretion of IL-4 protein induced by rHRF significantly correlated with histamine release and the amount of protein generated, and the kinetics were identical to those caused by anti-IgE activation. Furthermore, the ability of rHRF to induce IL-4 protein production from cells not normally responsive to this protein was transferred by passive sensitization with plasma containing IgE+ antibody. That this novel protein stimulates both mediator release and the secretion of IL-4 protein from human basophils suggests a prominent role for this molecule in allergic disease.

Original languageEnglish (US)
Pages (from-to)1265-1270
Number of pages6
JournalJournal of Experimental Medicine
Volume183
Issue number3
DOIs
StatePublished - Mar 1 1996

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Basophils
Interleukin-4
Immunoglobulin E
Proteins
Histamine Release
translationally-controlled 1 tumor protein
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

An immunoglobulin E-dependent recombinant histamine-releasing factor induces interleukin-4 secretion from human basophils. / Schroeder, John Thomas; Lichtenstein, Lawrence M.; MacDonald, Susan M.

In: Journal of Experimental Medicine, Vol. 183, No. 3, 01.03.1996, p. 1265-1270.

Research output: Contribution to journalArticle

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