An immunofluorescence procedure for the tissue localization of glucosephosphate isomerase

J. Gearhart, M. L. Oster-Granite

Research output: Contribution to journalArticle

Abstract

Anti-glucosephosphate isomerase antibodies have been used to reveal the presence of the glycolytic and gluconeogenic enzyme, glucosephosphate isomerase (GPI) in mouse tissues by an indirect immunofluorescence technique. The enzyme was localized in 6-μm sections from tissues embedded in paraffin. GPI was observed in the sarcoplasm of skeletal muscle, all hepatocytes of the liver, and in the granulosa cells and oocytes of growing ovarian follicles. The immunofluorescence procedure circumvents the problems associated with the conventional localization of GPI by histochemistry and appears to be a more sensitive procedure for the detection of low concentration of GPI, as demonstrated by the staining of the ovarian follicles.

Original languageEnglish (US)
Pages (from-to)245-249
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume28
Issue number3
StatePublished - 1980
Externally publishedYes

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Glucose-6-Phosphate Isomerase
Fluorescent Antibody Technique
Ovarian Follicle
Granulosa Cells
Enzymes
Indirect Fluorescent Antibody Technique
Paraffin
Oocytes
Hepatocytes
Skeletal Muscle
Staining and Labeling
Antibodies
Liver

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

An immunofluorescence procedure for the tissue localization of glucosephosphate isomerase. / Gearhart, J.; Oster-Granite, M. L.

In: Journal of Histochemistry and Cytochemistry, Vol. 28, No. 3, 1980, p. 245-249.

Research output: Contribution to journalArticle

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