TY - JOUR
T1 - An immunofluorescence procedure for the tissue localization of glucosephosphate isomerase
AU - Gearhart, J.
AU - Oster-Granite, M. L.
PY - 1980
Y1 - 1980
N2 - Anti-glucosephosphate isomerase antibodies have been used to reveal the presence of the glycolytic and gluconeogenic enzyme, glucosephosphate isomerase (GPI) in mouse tissues by an indirect immunofluorescence technique. The enzyme was localized in 6-μm sections from tissues embedded in paraffin. GPI was observed in the sarcoplasm of skeletal muscle, all hepatocytes of the liver, and in the granulosa cells and oocytes of growing ovarian follicles. The immunofluorescence procedure circumvents the problems associated with the conventional localization of GPI by histochemistry and appears to be a more sensitive procedure for the detection of low concentration of GPI, as demonstrated by the staining of the ovarian follicles.
AB - Anti-glucosephosphate isomerase antibodies have been used to reveal the presence of the glycolytic and gluconeogenic enzyme, glucosephosphate isomerase (GPI) in mouse tissues by an indirect immunofluorescence technique. The enzyme was localized in 6-μm sections from tissues embedded in paraffin. GPI was observed in the sarcoplasm of skeletal muscle, all hepatocytes of the liver, and in the granulosa cells and oocytes of growing ovarian follicles. The immunofluorescence procedure circumvents the problems associated with the conventional localization of GPI by histochemistry and appears to be a more sensitive procedure for the detection of low concentration of GPI, as demonstrated by the staining of the ovarian follicles.
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M3 - Article
C2 - 6986434
AN - SCOPUS:0018856396
SN - 0022-1554
VL - 28
SP - 245
EP - 249
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 3
ER -