Anti-glucosephosphate isomerase antibodies have been used to reveal the presence of the glycolytic and gluconeogenic enzyme, glucosephosphate isomerase (GPI) in mouse tissues by an indirect immunofluorescence technique. The enzyme was localized in 6-μm sections from tissues embedded in paraffin. GPI was observed in the sarcoplasm of skeletal muscle, all hepatocytes of the liver, and in the granulosa cells and oocytes of growing ovarian follicles. The immunofluorescence procedure circumvents the problems associated with the conventional localization of GPI by histochemistry and appears to be a more sensitive procedure for the detection of low concentration of GPI, as demonstrated by the staining of the ovarian follicles.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Histochemistry and Cytochemistry|
|State||Published - 1980|
ASJC Scopus subject areas
- Cell Biology