An experimental intratonsilar infection model for bovine tuberculosis in African buffaloes, Syncerus caffer

L. De Klerk, A. L. Michel, D. G. Grobler, R. G. Bengis, M. Bush, N. P.J. Kriek, M. S. Hofmeyr, J. F.T. Griffin, G. G. Mackintosh

Research output: Contribution to journalArticlepeer-review

Abstract

An infection model for Mycobacterium bovis in African buffaloes, Syncerus caffer, was developed, using the intratonsilar route of inoculation. Two groups of 11 buffaloes each, aged approximately 18 months, were infected with either 3.2 × 102 cfu (low dose) or 3 × 104 cfu (high dose) of M. bovis strain isolated from a buffalo. A control group of six buffaloes received saline via the same route. The infection status was monitored in vivo using the comparative intradermal tuberculin test, and in vitro by the modified interferon-gamma assay. All buffaloes were euthanazed 22 weeks post infection and lesion development was assessed by macroscopic examination, culture and histopathology. It was found that the high dose caused macroscopic lesions in nine out of 11 buffaloes. Mycobacterium bovis was isolated from all buffaloes in the high-dose group and from six out of 11 in the low-dose group.

Original languageEnglish (US)
Pages (from-to)293-303
Number of pages11
JournalOnderstepoort Journal of Veterinary Research
Volume73
Issue number4
StatePublished - Dec 2006

Keywords

  • African buffalo
  • Bacille Calmette-GuéRin
  • Bovine tuberculosis
  • Intratonsilar infection model Kruger National Park
  • Mycobacterium bovis
  • Syncerus caffer

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'An experimental intratonsilar infection model for bovine tuberculosis in African buffaloes, Syncerus caffer'. Together they form a unique fingerprint.

Cite this