An experiment-based algorithm for predicting the partitioning of unfolded peptides into phosphatidylcholine bilayer interfaces

Kalina A Hristova, Stephen H. White

Research output: Contribution to journalArticle

Abstract

Knowing the partitioning free energy of unfolded polypeptides into membrane interfaces is necessary for understanding membrane protein stability and for designing antimicrobial and other peptides. Experiment-based whole-residue free-energy (hydropathy) scales for amino acids in unfolded peptides, derived from the partitioning of host-guest pentapeptides (Ac-WLXLL) into the interfaces of phosphatidylcholine bilayers and into n-octanol, have been determined by W. C. Wimley, S. H. White, and colleagues [(1996) Nat. Struc. Biol. 3, 842; Wimley, W. C. et al. (1996) Biochemistry 35, 5109]. These scales offer the possibility of computing absolute partitioning free energies of unfolded peptides given only their amino acid sequences. However, the scales are incomplete, because partitioning free energies of N- and C-terminal groups are missing. To complete the scales, we have measured the pH-dependent partitioning of the host-guest pentapeptide variants AcWL-X-LL-NH2 and WL-X-LL-NH2 (X = G or W) into palmitoyloleoylphosphatidylcholine (POPC) bilayer interfaces and n-octanol. These measurements, in combination with the earlier ones, lead to hydrophobicity scale values for protonation, deprotonation, or acetylation of the N terminus and protonation, deprotonation, or amidation of the C terminus. A surprising finding is that a charged N terminus has a much smaller effect on bilayer partitioning than a charged C terminus. We present a simple algorithm for computing the absolute partitioning free energies of unfolded peptides into the phosphatidylcholine bilayer interface.

Original languageEnglish (US)
Pages (from-to)12614-12619
Number of pages6
JournalBiochemistry®
Volume44
Issue number37
DOIs
StatePublished - Sep 20 2005

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Phosphatidylcholines
Free energy
Peptides
1-Octanol
Deprotonation
Protonation
Experiments
Amino Acids
Acetylation
Biochemistry
Protein Stability
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Amino Acid Sequence
Membrane Proteins
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

An experiment-based algorithm for predicting the partitioning of unfolded peptides into phosphatidylcholine bilayer interfaces. / Hristova, Kalina A; White, Stephen H.

In: Biochemistry®, Vol. 44, No. 37, 20.09.2005, p. 12614-12619.

Research output: Contribution to journalArticle

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