An evaluation of the use of two-dimensional gel electrophoresis in proteomics

Shao En Ong, Akhilesh Pandey

Research output: Contribution to journalArticle

Abstract

With whole genomes being sequenced almost routinely, the next logical step towards a better understanding of cellular mechanisms lies in studying the functional units of gene expression - proteins. One of the fundamental approaches in proteomics is the use of two-dimensional gel electrophoresis as a mode of separation and visualization of complex protein mixtures. Despite several limitations of the method, its ability to separate large numbers of proteins, including their post-translationally modified forms, ensures that it will continue to be popular in several well-defined areas of proteomics. In this article, we discuss the merits and drawbacks of two-dimensional gels and compare them with alternative systems such as one-dimensional gels and liquid chromatography-based separation methods. In the wake of recent advances in mass spectrometry and related areas, we outline areas where two-dimensional gels can best be utilized as the preferred separation method in proteomic strategies.

Original languageEnglish (US)
Pages (from-to)195-205
Number of pages11
JournalBiomolecular Engineering
Volume18
Issue number5
DOIs
StatePublished - 2001

Keywords

  • Cellular mechanisms
  • Gel electrophoresis
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology

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