An essential role of mast cells in the development of airway hyperresponsiveness in a murine asthma model

Tetsuto Kobayashi, Toru Miura, Tomoko Haba, Miyuki Sato, Isao Serizawa, Hiroichi Nagai, Kimishige Ishizaka

Research output: Contribution to journalArticle

Abstract

Immunization of BALB/c mice with alum-adsorbed OVA, followed by three bronchoprovocations with aerosolized OVA, resulted in the development of airway hyperresponsiveness (AHR) and allergic inflammation in the lung accompanied by severe infiltration of eosinophils into airways. In this murine asthma model, administration of monoclonal anti-IL-5 Ab before each Ag challenge markedly inhibited airway eosinophilia, but the treatment did not affect the development of AHR. Immunization and aerosol challenges with OVA following the same protocol failed to induce AHR in the mast cell-deficient W/W(v) mice, but induced AHR in their congenic littermates, i.e., WBB6F1 (+/+) mice. No significant difference was found between the W/W(v) mice and +/+ mice with respect to the IgE and IgG1 anti-OVA Ab responses and to the airway eosinophilia after Ag provocations. It was also found that reconstitution of W/W(v) mice with bone marrow-derived mast cells cultured from normal littermates restored the capacity of developing Ag-induced AHR, indicating that lack of mast cells was responsible for the failure of W/W(v) mice to develop Ag-induced AHR under the experimental conditions. However, the OVA-immunized W/W(v) mice developed AHR by increasing the frequency and Ag dose of bronchoprovocations. The results suggested that AHR could be developed by two distinct cellular mechanisms. One would go through mast cell activation and the other is IgE/mast cell independent but an eosinophil/IL-5- dependent mechanism.

Original languageEnglish (US)
Pages (from-to)3855-3861
Number of pages7
JournalJournal of Immunology
Volume164
Issue number7
StatePublished - Apr 1 2000
Externally publishedYes

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Mast Cells
Asthma
Interleukin-5
Eosinophilia
Eosinophils
Immunization
Aerosols
Immunoglobulin E
Pneumonia
Immunoglobulin G
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

Kobayashi, T., Miura, T., Haba, T., Sato, M., Serizawa, I., Nagai, H., & Ishizaka, K. (2000). An essential role of mast cells in the development of airway hyperresponsiveness in a murine asthma model. Journal of Immunology, 164(7), 3855-3861.

An essential role of mast cells in the development of airway hyperresponsiveness in a murine asthma model. / Kobayashi, Tetsuto; Miura, Toru; Haba, Tomoko; Sato, Miyuki; Serizawa, Isao; Nagai, Hiroichi; Ishizaka, Kimishige.

In: Journal of Immunology, Vol. 164, No. 7, 01.04.2000, p. 3855-3861.

Research output: Contribution to journalArticle

Kobayashi, T, Miura, T, Haba, T, Sato, M, Serizawa, I, Nagai, H & Ishizaka, K 2000, 'An essential role of mast cells in the development of airway hyperresponsiveness in a murine asthma model', Journal of Immunology, vol. 164, no. 7, pp. 3855-3861.
Kobayashi T, Miura T, Haba T, Sato M, Serizawa I, Nagai H et al. An essential role of mast cells in the development of airway hyperresponsiveness in a murine asthma model. Journal of Immunology. 2000 Apr 1;164(7):3855-3861.
Kobayashi, Tetsuto ; Miura, Toru ; Haba, Tomoko ; Sato, Miyuki ; Serizawa, Isao ; Nagai, Hiroichi ; Ishizaka, Kimishige. / An essential role of mast cells in the development of airway hyperresponsiveness in a murine asthma model. In: Journal of Immunology. 2000 ; Vol. 164, No. 7. pp. 3855-3861.
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