An essential role for β-actin mRNA localization and translation in Ca2+-dependent growth cone guidance

Jiaqi Yao, Yukio Sasaki, Zhexing Wen, Gary J. Bassell, James Q. Zheng

Research output: Contribution to journalArticle

Abstract

Axon pathfinding requires directional responses of growth cones to extracellular cues, which have been shown to involve local synthesis of protein. The identity and functions of the locally produced proteins remain, however, unclear. Here we report that Ca2+-dependent bidirectional turning of Xenopus laevis growth cones requires localized distribution and translation of β-actin messenger RNA. Both β-actin mRNA and its zipcode-binding protein, ZBP1, are localized at the growth cone and become asymmetrically distributed upon local exposure to brain-derived neurotrophic factor (BDNF). Inhibition of protein synthesis or antisense interference with β-actin mRNA-ZBP1 binding abolishes both Ca2+-mediated attraction and repulsion. In addition, attraction involves a local increase in β-actin, whereas repulsion is accompanied by a local decrease in β-actin; thus, both produce a synthesis- and ZBP1 binding-dependent β-actin asymmetry but with opposite polarities. Together with a similar asymmetry in Src activity during bidirectional responses, our findings indicate that Ca2+-dependent spatial regulation of β-actin synthesis through Src contributes to the directional motility of growth cones during guidance.

Original languageEnglish (US)
Pages (from-to)1265-1273
Number of pages9
JournalNature neuroscience
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2006

ASJC Scopus subject areas

  • Neuroscience(all)

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