An epigenetic marker panel for recurrence risk prediction of low grade papillary urothelial cell carcinoma (LGPUCC) and its potential use for surveillance after transurethral resection using urine

Leonel Maldonado, Mariana Brait Rodrigues De Oliveira, Christina Michailidi, Enrico Munari, Tina Driscoll, Luciana Schultz, Trinity Bivalacqua, Mark Schoenberg, David Sidransky, George J. Netto, Mohammad Hoque

Research output: Contribution to journalArticle

Abstract

By a candidate gene approach, we analyzed the promoter methylation (PM) of 8 genes (ARF, TIMP3, RAR-β2, NID2, CCNA1, AIM1, CALCA and CCND2) by quantitative methylation specific PCR (QMSP) in the DNA of 17 non-recurrent and 19 recurrent noninvasive low grade papillary urothelial cell carcinoma (LGPUCC) archival tissues. Among the genes tested, by establishing an empiric cutoff value, CCND2, CCNA1, NID2, and CALCA showed higher frequency of methylation in recurrent than in non-recurrent LGPUCC: CCND2 10/19 (53%) vs. 2/17 (12%) (p=0.014); CCNA1 11/19 (58%) vs. 4/17 (23.5%) (p=0.048); NID2 13/19 (68%) vs. 3/17 (18%) (p=0.003) and CALCA 10/19 (53%) vs. 4/17 (23.5%) (p=0.097), respectively. We further analyzed PM of CCND2, CCNA1, and CALCA in urine DNA from UCC patients including LGPUCC and controls. The frequency of CCND2, CCNA1, and CALCA was significantly higher (p

Original languageEnglish (US)
Pages (from-to)5218-5233
Number of pages16
JournalOncotarget
Volume5
Issue number14
StatePublished - 2014

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Epigenomics
Methylation
Urine
Carcinoma
Recurrence
Genes
DNA
Polymerase Chain Reaction

Keywords

  • Biomarkers
  • DNA methylation
  • Epigenetics
  • LGPUCC
  • Recurrence

ASJC Scopus subject areas

  • Oncology

Cite this

An epigenetic marker panel for recurrence risk prediction of low grade papillary urothelial cell carcinoma (LGPUCC) and its potential use for surveillance after transurethral resection using urine. / Maldonado, Leonel; Brait Rodrigues De Oliveira, Mariana; Michailidi, Christina; Munari, Enrico; Driscoll, Tina; Schultz, Luciana; Bivalacqua, Trinity; Schoenberg, Mark; Sidransky, David; Netto, George J.; Hoque, Mohammad.

In: Oncotarget, Vol. 5, No. 14, 2014, p. 5218-5233.

Research output: Contribution to journalArticle

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AU - Michailidi, Christina

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AU - Driscoll, Tina

AU - Schultz, Luciana

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