TY - JOUR
T1 - An enzymatic method for 5-fluorocytosine
AU - Huang, C. M.
AU - Kroll, M. H.
AU - Ruddel, M.
AU - Washburn, R. G.
AU - Bennett, J. E.
PY - 1988
Y1 - 1988
N2 - We developed an enzymatic method for determination of 5-fluorocytosine in serum, using creatine iminohydrolase (EC 3.5.4.21), the Cobas-Bio analyzer, and an extant ammonia method. Analytical recovery (y) of drug added to serum (x) was good, with y = 0.97x - 0.7, S(y·x) = 3.6, r = 0.997 (n = 65) over the range 6.25 to 150 mg/L. Comparison with and HPLC method (x) showed good agreement: y = 0.98x + 1.34, S(y·x) = 3.7 (n = 37), as analyzed with the Deming debiased regression. Precision was good, CVs being <3% for within-run and <6% for between-run controls. Ammonia, amphotericin B, glucose, urea, and hemolysis do not interfere, but bilirubin shows analyte-dependent interference and lipemia interferes when triglycerides exceed 5 g/L. This assay is accurate, inexpensive, and easy to perform. It can be easily adapted for routine or emergency use.
AB - We developed an enzymatic method for determination of 5-fluorocytosine in serum, using creatine iminohydrolase (EC 3.5.4.21), the Cobas-Bio analyzer, and an extant ammonia method. Analytical recovery (y) of drug added to serum (x) was good, with y = 0.97x - 0.7, S(y·x) = 3.6, r = 0.997 (n = 65) over the range 6.25 to 150 mg/L. Comparison with and HPLC method (x) showed good agreement: y = 0.98x + 1.34, S(y·x) = 3.7 (n = 37), as analyzed with the Deming debiased regression. Precision was good, CVs being <3% for within-run and <6% for between-run controls. Ammonia, amphotericin B, glucose, urea, and hemolysis do not interfere, but bilirubin shows analyte-dependent interference and lipemia interferes when triglycerides exceed 5 g/L. This assay is accurate, inexpensive, and easy to perform. It can be easily adapted for routine or emergency use.
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U2 - 10.1093/clinchem/34.1.59
DO - 10.1093/clinchem/34.1.59
M3 - Article
C2 - 3338187
AN - SCOPUS:0023839741
SN - 0009-9147
VL - 34
SP - 59
EP - 62
JO - Clinical chemistry
JF - Clinical chemistry
IS - 1
ER -