An enzymatic method for 5-fluorocytosine

C. M. Huang, M. H. Kroll, M. Ruddel, R. G. Washburn, J. E. Bennett

Research output: Contribution to journalArticle

Abstract

We developed an enzymatic method for determination of 5-fluorocytosine in serum, using creatine iminohydrolase (EC 3.5.4.21), the Cobas-Bio analyzer, and an extant ammonia method. Analytical recovery (y) of drug added to serum (x) was good, with y = 0.97x - 0.7, S(y·x) = 3.6, r = 0.997 (n = 65) over the range 6.25 to 150 mg/L. Comparison with and HPLC method (x) showed good agreement: y = 0.98x + 1.34, S(y·x) = 3.7 (n = 37), as analyzed with the Deming debiased regression. Precision was good, CVs being

Original languageEnglish (US)
Pages (from-to)59-62
Number of pages4
JournalClinical Chemistry
Volume34
Issue number1
StatePublished - 1988
Externally publishedYes

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creatinine deiminase
Flucytosine
Ammonia
Recovery
Pharmaceutical Preparations
Serum
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Huang, C. M., Kroll, M. H., Ruddel, M., Washburn, R. G., & Bennett, J. E. (1988). An enzymatic method for 5-fluorocytosine. Clinical Chemistry, 34(1), 59-62.

An enzymatic method for 5-fluorocytosine. / Huang, C. M.; Kroll, M. H.; Ruddel, M.; Washburn, R. G.; Bennett, J. E.

In: Clinical Chemistry, Vol. 34, No. 1, 1988, p. 59-62.

Research output: Contribution to journalArticle

Huang, CM, Kroll, MH, Ruddel, M, Washburn, RG & Bennett, JE 1988, 'An enzymatic method for 5-fluorocytosine', Clinical Chemistry, vol. 34, no. 1, pp. 59-62.
Huang CM, Kroll MH, Ruddel M, Washburn RG, Bennett JE. An enzymatic method for 5-fluorocytosine. Clinical Chemistry. 1988;34(1):59-62.
Huang, C. M. ; Kroll, M. H. ; Ruddel, M. ; Washburn, R. G. ; Bennett, J. E. / An enzymatic method for 5-fluorocytosine. In: Clinical Chemistry. 1988 ; Vol. 34, No. 1. pp. 59-62.
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