TY - JOUR
T1 - An enzymatic-isotopic microassay for putrescine
AU - Harik, Sami I.
AU - Pasternak, Gavril W.
AU - Snyder, Solomon H.
N1 - Funding Information:
This work was supported by U.S.P.H.S.N.I.H. research grants MH-18501, MH-23211 and NS-07275. S. I. H. is supported by the Commonwealth Fund Exchange Fellowship Program at the Johns Hopkins University. G. W. P. is supported by The Insurance Medical Scientist Scholarship Fund; Mutual of Omaha Insurance Company and United Benefit Life Insurance Company. S. H. S. is recipient of N.I.M.H. Research Scientist Development Award MH-33128.
PY - 1973/5/28
Y1 - 1973/5/28
N2 - A highly sensitive enzymatic isotopic microassay procedure for the measurement of putrescine (1,4-diaminobutane) is described. The method depends on the enhancement by putrescine of the decarboxylation of S-adenosyl-L-[carboxy-14C] methionine by baker's yeast (Saccharomyces cerevisiae) S-adenosyl-L-methionine decarboxylase in the presence of varying amounts of putrescine. The quantity of 14CO2 evolved is a linear function of the amount of putrescine present. This method was used to measure the putrescine content of various tissues.
AB - A highly sensitive enzymatic isotopic microassay procedure for the measurement of putrescine (1,4-diaminobutane) is described. The method depends on the enhancement by putrescine of the decarboxylation of S-adenosyl-L-[carboxy-14C] methionine by baker's yeast (Saccharomyces cerevisiae) S-adenosyl-L-methionine decarboxylase in the presence of varying amounts of putrescine. The quantity of 14CO2 evolved is a linear function of the amount of putrescine present. This method was used to measure the putrescine content of various tissues.
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U2 - 10.1016/0304-4165(73)90222-5
DO - 10.1016/0304-4165(73)90222-5
M3 - Article
C2 - 4580200
AN - SCOPUS:0015938276
VL - 304
SP - 753
EP - 764
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 3
ER -