An enzymatic-isotopic microassay for putrescine

Sami I. Harik, Gavril W. Pasternak, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

A highly sensitive enzymatic isotopic microassay procedure for the measurement of putrescine (1,4-diaminobutane) is described. The method depends on the enhancement by putrescine of the decarboxylation of S-adenosyl-L-[carboxy-14C] methionine by baker's yeast (Saccharomyces cerevisiae) S-adenosyl-L-methionine decarboxylase in the presence of varying amounts of putrescine. The quantity of 14CO2 evolved is a linear function of the amount of putrescine present. This method was used to measure the putrescine content of various tissues.

Original languageEnglish (US)
Pages (from-to)753-764
Number of pages12
JournalBBA - General Subjects
Volume304
Issue number3
DOIs
StatePublished - May 28 1973

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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