An enzymatic-isotopic microassay for putrescine

Sami I. Harik; Gavril W. Pasternak; Solomon H. Snyder

doi:10.1016/0304-4165(73)90222-5

An enzymatic-isotopic microassay for putrescine

Sami I. Harik, Gavril W. Pasternak, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article

Abstract

A highly sensitive enzymatic isotopic microassay procedure for the measurement of putrescine (1,4-diaminobutane) is described. The method depends on the enhancement by putrescine of the decarboxylation of S-adenosyl-L-[carboxy-¹⁴C] methionine by baker's yeast (Saccharomyces cerevisiae) S-adenosyl-L-methionine decarboxylase in the presence of varying amounts of putrescine. The quantity of ¹⁴CO₂ evolved is a linear function of the amount of putrescine present. This method was used to measure the putrescine content of various tissues.

Original language	English (US)
Pages (from-to)	753-764
Number of pages	12
Journal	BBA - General Subjects
Volume	304
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(73)90222-5
State	Published - May 28 1973

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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Harik, S. I., Pasternak, G. W., & Snyder, S. H. (1973). An enzymatic-isotopic microassay for putrescine. BBA - General Subjects, 304(3), 753-764. https://doi.org/10.1016/0304-4165(73)90222-5

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AB - A highly sensitive enzymatic isotopic microassay procedure for the measurement of putrescine (1,4-diaminobutane) is described. The method depends on the enhancement by putrescine of the decarboxylation of S-adenosyl-L-[carboxy-14C] methionine by baker's yeast (Saccharomyces cerevisiae) S-adenosyl-L-methionine decarboxylase in the presence of varying amounts of putrescine. The quantity of 14CO2 evolved is a linear function of the amount of putrescine present. This method was used to measure the putrescine content of various tissues.

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