An endogenous morphine-like factor in mammalian brain

Gavril W. Pasternak; Robert Goodman; Solomon H. Snyder

doi:10.1016/0024-3205(75)90270-2

An endogenous morphine-like factor in mammalian brain

Gavril W. Pasternak, Robert Goodman, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

273 Scopus citations

Abstract

An endogenous morphine-like substance (MLF) found in rat and calf brains has a regional distribution correlating with that of opiate receptors, with the highest levels in the caudate and negligible amounts in the cerebellum. In binding assays MLF behaves like an opiate agonist. Sodium ion and enzyme and reagent treatment of membranes decrease its potency and manganese ion enhances it. MLF is localized in synaptosomal fractions, stored in an osmotically labile compartment, and can be degraded by carboxypeptidase A and leucine amino peptidase, implying a peptide structure. Its molecular weight is about 1000 as determined by gel chromatography.

Original language	English (US)
Pages (from-to)	1765-1769
Number of pages	5
Journal	Life Sciences
Volume	16
Issue number	12
DOIs	https://doi.org/10.1016/0024-3205(75)90270-2
State	Published - Jun 15 1975

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(75)90270-2

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abstract = "An endogenous morphine-like substance (MLF) found in rat and calf brains has a regional distribution correlating with that of opiate receptors, with the highest levels in the caudate and negligible amounts in the cerebellum. In binding assays MLF behaves like an opiate agonist. Sodium ion and enzyme and reagent treatment of membranes decrease its potency and manganese ion enhances it. MLF is localized in synaptosomal fractions, stored in an osmotically labile compartment, and can be degraded by carboxypeptidase A and leucine amino peptidase, implying a peptide structure. Its molecular weight is about 1000 as determined by gel chromatography.",

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AU - Pasternak, Gavril W.

AU - Goodman, Robert

AU - Snyder, Solomon H.

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N2 - An endogenous morphine-like substance (MLF) found in rat and calf brains has a regional distribution correlating with that of opiate receptors, with the highest levels in the caudate and negligible amounts in the cerebellum. In binding assays MLF behaves like an opiate agonist. Sodium ion and enzyme and reagent treatment of membranes decrease its potency and manganese ion enhances it. MLF is localized in synaptosomal fractions, stored in an osmotically labile compartment, and can be degraded by carboxypeptidase A and leucine amino peptidase, implying a peptide structure. Its molecular weight is about 1000 as determined by gel chromatography.

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An endogenous morphine-like factor in mammalian brain

Abstract

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