An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells

Toshie Kai, Allan Spradling

Research output: Contribution to journalArticlepeer-review

Abstract

Stem cells are thought to reside in regulatory microenvironments ("niches") generated by stable stromal neighbors. To investigate the significance of empty niches vacated by stem cell loss, we studied Drosophila ovarioles, which maintain two to three germline stem cells in a niche requiring adhesive stromal cap cells and Decapentaplegic signals. After experimentally emptying the germline stem cell niche, cap cell activity persists for several weeks. Initially, somatic inner germarium sheath cells enter the empty niche, respond to Dpp, but fail to divide. Subsequently, follicle cell progenitors, including somatic stem cells enter the niche, respond to Dpp, and proliferate as long as cap cells remain. Proliferation requires the normal hedgehog signal of the somatic stem cells as well as proximity to the niche. Thus, empty niches can persist, signal incoming cells, and support ectopic proliferation. Similar events may underlie some disease states.

Original languageEnglish (US)
Pages (from-to)4633-4638
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number8
DOIs
StatePublished - Apr 15 2003
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • General

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