TY - JOUR
T1 - An autoradiographic study of α1-adrenergic receptors in the brain of the Japanese quail (Coturnix coturnix japonica)
AU - Balthazart, Jacques
AU - Ball, Gregory F.
AU - McEwen, Bruce S.
PY - 1989/12
Y1 - 1989/12
N2 - The neuroanatomical distribution of α1-adrenergic receptors was studied in Japanese quail by quantitative in vitro autoradiography using the specific antagonist [3H]prazosin as the ligand. The presence of saturable (Bmax <200 fmol/mg protein) high affinity (Kd < 0.12 nM) binding sites was detected by saturation analysis. High concentrations of [3H]prazosin binding sites were detected in the archistriatum/pars ventralis, the hippocampus, the cortex piriformis, the area corticoidea dorsolateralis, the dorsal thalamus, and the nucleus praetectalis. Lower concentrations were seen in the intercollicular nucleus, the lateral septum, and the posterior and tuberal hypothalamus. Very little binding was seen in the preoptic and anterior hypothalamic areas. The relatively high number of binding sites identified in the telencephalic structures agrees well with previous mammalian studies. This is in contrast with the pattern in the anterior hypothalamus where, in mammals, a number of nuclei have been reported to contain a high receptor density.
AB - The neuroanatomical distribution of α1-adrenergic receptors was studied in Japanese quail by quantitative in vitro autoradiography using the specific antagonist [3H]prazosin as the ligand. The presence of saturable (Bmax <200 fmol/mg protein) high affinity (Kd < 0.12 nM) binding sites was detected by saturation analysis. High concentrations of [3H]prazosin binding sites were detected in the archistriatum/pars ventralis, the hippocampus, the cortex piriformis, the area corticoidea dorsolateralis, the dorsal thalamus, and the nucleus praetectalis. Lower concentrations were seen in the intercollicular nucleus, the lateral septum, and the posterior and tuberal hypothalamus. Very little binding was seen in the preoptic and anterior hypothalamic areas. The relatively high number of binding sites identified in the telencephalic structures agrees well with previous mammalian studies. This is in contrast with the pattern in the anterior hypothalamus where, in mammals, a number of nuclei have been reported to contain a high receptor density.
KW - Coturnix coturnix japonica (Aves, Phasianiformes)
KW - Japanese quail
KW - Noradrenergic system
KW - Prazosin
KW - Quantitative autoradiography
KW - α-Adrenergic receptor
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U2 - 10.1007/BF00218868
DO - 10.1007/BF00218868
M3 - Article
C2 - 2558805
AN - SCOPUS:0024306267
VL - 258
SP - 563
EP - 568
JO - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
JF - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
SN - 0302-766X
IS - 3
ER -