An anchored restriction-mapping approach applied to the genetic analysis of the Anopheles gambiae malaria vector complex 1

Kostas D. Mathiopoulos, Jeffrey D. Powell, Thomas F. McCutchan

Research output: Contribution to journalArticle

Abstract

We introduce here a simple approach for rapidly determining restriction maps for a number of regions of a genome; this involves 'anchoring' a map with a rare restriction site (in this case the seldom-cutting EagI) followed by partial digestion of a frequent-cutting enzyme (e.g., Sau3A). We applied this technology to five species of the Anopheles gambiae complex. In a single Southern blot we obtained about a 15-kb restriction map each for the mtDNA, rRNA gene, and a scnDNA region for each of five species. Phylogenetic analyses of these regions yield trees at odds with the more traditional chromosome inversion-based trees. The value of the approach for systematic purposes is the case with which several large, independent regions of the genome can be quickly assayed for molecular variation.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalMolecular biology and evolution
Volume12
Issue number1
DOIs
StatePublished - Jan 1995

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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