An analysis of the integration between articular cartilage and nondegradable hydrogel using magnetic resonance imaging

Sharan Ramaswamy, Dong An Wang, Kenneth W. Fishbein, Jennifer Hartt Elisseeff, Richard G. Spencer

Research output: Contribution to journalArticle

Abstract

A hydrogel is a highly hydrated polymer gel suitable for use as a scaffold for tissue engineering. One important application is to the repair of cartilage defects due to injury or osteoarthritis. Integration of the hydrogel with surrounding tissue is critical for the long-term functionality of the implant; however direct visualization of integration is difficult and invasive. Accordingly, we used MRI to noninvasively investigate the integration of hydrogel in cartilage. Two integration methods were assessed: (1) cartilage-initiated and (2) chemical, using chondroitin sulphate-methacrylate- aldehyde (CS-MA-ald) as an adhesive. These were compared to a control group, that is, standard, nonintegrated hydrogel photopolymerization. Spatial variation of the transverse relaxation time, T2, across the transition region was used to determine the effectiveness of integration. In the CS-MA-ald group only, two interfaces were found. This provides evidence of an intermediate adhesive layer between hydrogel and cartilage. Second, the thickness of the transition region between hydrogel to cartilage in the CS-MA-ald group was 1.32 mm as compared to 1.20 mm and 1.17 mm in the tissue-initiated and nonintegrated groups, respectively. We interpret this as a more gradual transition region of hydrogel to cartilage and hence a greater degree of integration when an adhesive layer is present.

Original languageEnglish (US)
Pages (from-to)144-148
Number of pages5
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume77
Issue number1
DOIs
StatePublished - Apr 2006

Fingerprint

Hydrogel
Cartilage
Magnetic resonance
Hydrogels
Imaging techniques
Methacrylates
Chondroitin Sulfates
Aldehydes
Adhesives
Tissue
Photopolymerization
Scaffolds (biology)
Tissue engineering
Magnetic resonance imaging
Relaxation time
Polymers
Repair
Gels
Visualization
Defects

Keywords

  • Cartilage
  • Hydrogel
  • Integration
  • Magnetic resonsnce imaging (MRI)
  • Transverse relaxation time (T)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

An analysis of the integration between articular cartilage and nondegradable hydrogel using magnetic resonance imaging. / Ramaswamy, Sharan; Wang, Dong An; Fishbein, Kenneth W.; Elisseeff, Jennifer Hartt; Spencer, Richard G.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 77, No. 1, 04.2006, p. 144-148.

Research output: Contribution to journalArticle

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