Abstract
Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be analyzed by mass spectrometry imaging.
Original language | English (US) |
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Pages (from-to) | 1885-1890 |
Number of pages | 6 |
Journal | Journal of the American Society for Mass Spectrometry |
Volume | 22 |
Issue number | 10 |
DOIs | |
State | Published - Oct 2011 |
Keywords
- Imaging mass spectrometry
- MALDI
- Peptides and proteins
- SIMS
- Washing
ASJC Scopus subject areas
- Structural Biology
- Spectroscopy