An all-in-one microfluidic device for parallel DNA extraction and gene analysis

Yi Zhang, Seungkyung Park, Samuel Yang, Tza Huei Wang

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a microfluidic device capable of fully integrated sample preparation and gene analysis from crude biosamples such as whole blood. Our platform takes the advantage of the silica superparamagnetic particle based solid phase extraction to develop an all-in-one scheme that performs cell lysis, DNA binding, washing, elution and the PCR in the same reaction chamber. The device also employs a unique reagent loading scheme, allowing efficient preparation of multiple reactions via a single injection channel. In addition, PCR is performed in a droplet-in-oil manner, eliminating the need for chamber sealing. The combination of these design features greatly reduces the complexity in implementing fully integrated lab-on-a-chip systems for genetic detection, facilitating parallel analysis of multiple samples or genes on a single microchip. The capability of the device is demonstrated by performing DNA isolation from the human whole blood sample and analyzing the Rsf-1 gene using the TaqMan probe based gene specific PCR assays.

Original languageEnglish (US)
Pages (from-to)1043-1049
Number of pages7
JournalBiomedical microdevices
Volume12
Issue number6
DOIs
StatePublished - Dec 2010

Keywords

  • All-in-one
  • PCR
  • PDMS
  • Sample preparation
  • Silica superparamagnetic particles
  • Solid phase extraction

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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