TY - JOUR
T1 - An aedes aegypti-associated fungus increases susceptibility to dengue virus by modulating gut trypsin activity
AU - Angleró-Rodríguez, Yesseinia I.
AU - Talyuli, Octavio A.C.
AU - Blumberg, Benjamin J.
AU - Kang, Seokyoung
AU - Demby, Celia
AU - Shields, Alicia
AU - Carlson, Jenny
AU - Jupatanakul, Natapong
AU - Dimopoulos, George
N1 - Funding Information:
We would like to thank the Johns Hopkins Malaria Research Institute Insectary and the Microarray Core Facilities. We also thank Dr. Deborah McClellan for editing the manuscript. We thank for the support with the statistical analysis from the National Center for Research Resources and the National Center for Advancing Translational Sciences (NCATS) of the National Institutes of Health through Grant Number 1UL1TR001079. This work has been supported by National Institutes of Health, National Institute of Allergy and Infectious Diseases grant R01AI101431. We thank the Bloomberg Philanthropies for their support.
Publisher Copyright:
© Angleró-Rodríguez et al.
PY - 2017/12/5
Y1 - 2017/12/5
N2 - Transmission of dengue virus (DENV) requires successful completion of the infection cycle in the Aedes aegypti vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. While certain Ae. aegypti midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility to DENV infection. We show that a Talaromyces (Tsp_PR) fungus, isolated from field-caught Ae. aegypti, render the mosquito more permissive to DENV infection. This modulation is attributed to a profound down-regulation of digestive enzyme genes and trypsin activity, upon exposure to Tsp_PR-secreted factors. In conclusion, we show for the first time that a natural mosquito gut-associated fungus can alter Ae. aegypti physiology in a way that facilitates pathogen infection.
AB - Transmission of dengue virus (DENV) requires successful completion of the infection cycle in the Aedes aegypti vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. While certain Ae. aegypti midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility to DENV infection. We show that a Talaromyces (Tsp_PR) fungus, isolated from field-caught Ae. aegypti, render the mosquito more permissive to DENV infection. This modulation is attributed to a profound down-regulation of digestive enzyme genes and trypsin activity, upon exposure to Tsp_PR-secreted factors. In conclusion, we show for the first time that a natural mosquito gut-associated fungus can alter Ae. aegypti physiology in a way that facilitates pathogen infection.
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U2 - 10.7554/eLife.28844
DO - 10.7554/eLife.28844
M3 - Article
C2 - 29205153
AN - SCOPUS:85040925146
SN - 2050-084X
VL - 6
JO - eLife
JF - eLife
M1 - e28844
ER -