Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats

Hossein Mirmiranpour, Shahnaz Khaghani, Ali Zandieh, O. Khalilzadeh, Siavash Gerayesh-Nejad, Afsaneh Morteza, Alireza Esteghamati

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Angiogenesis contributes to different physiological and pathological conditions. The aim of this study was to investigate for the first time the antiangiogenic effects of amygdalin on the cultured endothelial cells of diabetic rats. Materials and Methods: A total of 20 streptozotocin-induced diabetic rats were divided into two equal groups of control and amygdalin-treated animals. Eight weeks after the induction of diabetes, amygdalin was injected intraperitoneally (3 mg/kg) to the rats of the treatment group. One day later, rats were sacrificed; the aortic arteries were excised and cut as 2 mm rings. Each aortic ring was incubated in a cell-culture well for 7 days. The process of angiogenesis was monitored by counting the number of microvessels and primary microtubules in each well. Results: Optic microscopy showed proliferation and migration of new endothelial cells to the fibrin gels. The endothelial cells produced primary microtubules which gradually made several branches and finally made a vascular matrix. The number of the primary microtubules and microvessels were significantly lower in the amygdalin-treated vs. control group (P < 0.01). Conclusion: Therefore, amygdalin exerts inhibitory effects on angiogenesis in aortic rings of diabetic rats and may pave a new way for treatment of unfavorable angiogenic conditions.

Original languageEnglish (US)
Pages (from-to)211-214
Number of pages4
JournalIndian Journal of Pathology and Microbiology
Volume55
Issue number2
DOIs
StatePublished - Apr 2012
Externally publishedYes

Keywords

  • Amygdalin
  • Angiogenesis
  • Diabetes
  • Endothelial cells
  • Rat′s aortic rings

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Amygdalin inhibits angiogenesis in the cultured endothelial cells of diabetic rats'. Together they form a unique fingerprint.

Cite this