TY - JOUR
T1 - Amplifying undetectable NMR signals to study host-guest interactions and exchange
AU - Avram, Liat
AU - Iron, Mark A.
AU - Bar-Shir, Amnon
N1 - Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2016
Y1 - 2016
N2 - The characteristics of host-guest systems, such as molecular recognition, complexation, encapsulation, guest composition, and dynamic exchange, are manifested by changes in the chemical shifts (Δω) in the NMR spectrum. However, in cases where NMR signals cannot be detected, due to low concentrations, poor solubility, or relatively fast exchange, an alternative is needed. Here, we show that by using the magnetization transfer (MT) method, the undetectable NMR signals of host-guest assemblies can be amplified by two orders of magnitude. It is shown that the binding kinetics characteristics of a fluorinated guest and cucurbit[n]uril (CB[n]) hosts in aqueous solutions determine the NMR signal amplification of host-guest assemblies. In addition, by using the MT technique within the 19F-NMR framework, one can detect μM concentrations of the complex and study the effect of different solutes on the resulting host-guest system. The results expand the “NMR toolbox” available to explore a wider range of dynamic host-guest systems in which NMR signals cannot be detected.
AB - The characteristics of host-guest systems, such as molecular recognition, complexation, encapsulation, guest composition, and dynamic exchange, are manifested by changes in the chemical shifts (Δω) in the NMR spectrum. However, in cases where NMR signals cannot be detected, due to low concentrations, poor solubility, or relatively fast exchange, an alternative is needed. Here, we show that by using the magnetization transfer (MT) method, the undetectable NMR signals of host-guest assemblies can be amplified by two orders of magnitude. It is shown that the binding kinetics characteristics of a fluorinated guest and cucurbit[n]uril (CB[n]) hosts in aqueous solutions determine the NMR signal amplification of host-guest assemblies. In addition, by using the MT technique within the 19F-NMR framework, one can detect μM concentrations of the complex and study the effect of different solutes on the resulting host-guest system. The results expand the “NMR toolbox” available to explore a wider range of dynamic host-guest systems in which NMR signals cannot be detected.
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U2 - 10.1039/C6SC04083G
DO - 10.1039/C6SC04083G
M3 - Article
AN - SCOPUS:84995543066
VL - 7
SP - 6905
EP - 6909
JO - Chemical Science
JF - Chemical Science
SN - 2041-6520
IS - 12
ER -