TY - JOUR
T1 - Amplified DNA in Y1 mouse adrenal tumor cells
T2 - Isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6
AU - George, Donna L.
AU - Scott, Alan F.
AU - de Martinville, Bérengère
AU - Francke, Uta
N1 - Funding Information:
ACKNOWLEDGEMENTS This work was supported by NIH Research Grants CA-34462 (to DLG), GM-26105 (to UF), and GM-28931 (to AFS). EBS, EAS and EZS hybrids had been produced in the laboratory of Dr. John Minna. Expert research assistance was provided by V.E. Powers, P. Tetri, B. Foellmer, P. Clark, P. Hodges, M. Liao and S. Trusko.
PY - 1984/3/26
Y1 - 1984/3/26
N2 - We have isolated cDNA clones complementary to a c-Ki-ras cellular oncogene that is amplified in Yl mouse adrenal tumor cells, with the amplified sequences located on double-minute chromatin bodies (DMs) and homogeneously staining chromosomal regions (HSRs). Characterization of the cDNAs included the isolation of corresponding genomic clones, Northern blot analysis of RNA, and DNA sequence analysis. Our studies demonstrate that the c-Ki-ras gene amplified in the Yl cells is homologous to the human c-Ki-ras2 gene. We have also obtained evidence that, in addition to c-Ki-ras, at least one other transcription unit has been amplified in the mouse adrenal tumor cells. Moreover, by Southern blot analysis of Chinese hamster-mouse somatic cell hybrids, we have determined that the amplified DNA sequences associated with DMs and HSRs, including the c-Ki-ras gene, are present in normal mouse cells on chromosome 6
AB - We have isolated cDNA clones complementary to a c-Ki-ras cellular oncogene that is amplified in Yl mouse adrenal tumor cells, with the amplified sequences located on double-minute chromatin bodies (DMs) and homogeneously staining chromosomal regions (HSRs). Characterization of the cDNAs included the isolation of corresponding genomic clones, Northern blot analysis of RNA, and DNA sequence analysis. Our studies demonstrate that the c-Ki-ras gene amplified in the Yl cells is homologous to the human c-Ki-ras2 gene. We have also obtained evidence that, in addition to c-Ki-ras, at least one other transcription unit has been amplified in the mouse adrenal tumor cells. Moreover, by Southern blot analysis of Chinese hamster-mouse somatic cell hybrids, we have determined that the amplified DNA sequences associated with DMs and HSRs, including the c-Ki-ras gene, are present in normal mouse cells on chromosome 6
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U2 - 10.1093/nar/12.6.2731
DO - 10.1093/nar/12.6.2731
M3 - Article
C2 - 6546797
AN - SCOPUS:0021771158
SN - 0305-1048
VL - 12
SP - 2731
EP - 2743
JO - Nucleic acids research
JF - Nucleic acids research
IS - 6
ER -