Amplification of the c-myc Gene in Human Medulloblastoma Cell Lines and Xenografts

Darell D. Bigner, Sandra H. Bigner, Henry S. Friedman, W. Jerry Oakes, Bert Vogelstein

Research output: Contribution to journalArticle

Abstract

Cultured cell lines and xenografts derived from 7 human medulloblastomas were evaluated for amplification of the c-myc, N-myc, epidermal growth factor receptor, and gli genes by Southern blot analysis. Karyotypes of the original biopsies and early passaged cells demonstrated double minute chromosomes in 4 of the 7 cases. All 7 samples (3 cell lines and 4 xenografts) from the 4 tumors with double minute chromosomes contained amplification of the c-myc gene. Cell lines and xenografts derived from the 3 biopsies without double minute chromosomes failed to demonstrate amplification of the 4 genes which were tested, but a rearrangement of the c-myc gene occurred in 1 of the 3 tumors. These observations demonstrate that the c-myc gene is often amplified and/or rearranged in human medulloblastomas and suggest that amplification of this gene provides a growth advantage for medulloblastoma cells in vitro and fit vivo.

Original languageEnglish (US)
Pages (from-to)2347-2350
Number of pages4
JournalCancer Research
Volume50
Issue number8
StatePublished - Apr 15 1990

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Amplification of the c-myc Gene in Human Medulloblastoma Cell Lines and Xenografts'. Together they form a unique fingerprint.

  • Cite this

    Bigner, D. D., Bigner, S. H., Friedman, H. S., Oakes, W. J., & Vogelstein, B. (1990). Amplification of the c-myc Gene in Human Medulloblastoma Cell Lines and Xenografts. Cancer Research, 50(8), 2347-2350.