Amperometric microbiosensors based on PQQ-dependent glucose dehydrogenase towards the development of an ATP biosensor for in vitro analysis

C. Weber, E. Gauda, E. Hecht, B. Mizaikoff, C. Kranz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An amperometric biosensor for adenosine-5′- triphosphate (ATP) was developed applying a competitive assay of two glucose converting enzymes. Hexokinase and pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase were entrapped by pH-shift induced deposition of Resydrol at a platinum microelectrode with a diameter of 25 μm. Different mediators for the PQQ-dependent glucose deydrogenase (GDH) catalyzed reaction were used applying potentials not higher than 400 mV vs. Ag/AgCl reference electrode. The obtained biosensor is selective to ATP and sensitive to it at physiological concentrations down to the nanomolar range.

Original languageEnglish (US)
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Subtitle of host publicationMicro- and Nanosystems in Medicine, Active Implants, Biosensors
PublisherSpringer Verlag
Pages351-354
Number of pages4
Edition8
ISBN (Print)9783642038860
DOIs
StatePublished - 2009
EventWorld Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors - Munich, Germany
Duration: Sep 7 2009Sep 12 2009

Publication series

NameIFMBE Proceedings
Number8
Volume25
ISSN (Print)1680-0737

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Micro- and Nanosystems in Medicine, Active Implants, Biosensors
CountryGermany
CityMunich
Period9/7/099/12/09

Keywords

  • ATP
  • Hexokinase
  • Microbiosensor
  • PQQ-GDH
  • Resydrol

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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