Serum Aldolase was determined by measuring the rate of decrease in the current generated by NADH at a platinum electrode. NADH is the cofactor for a dehydrogenase enzyme in the coupled enzyme reaction scheme used in the determination. Reducing agents in reconstituted lyophilized human serum did not interfere with the determination. Serum aldolase was accurately determined between 4 and 14 mU/ml which is the normal range in a healthy individual. The proposed amperometric method compares quite favorably with the spectrophotometric method.
- Aldolase Triosephosphate Isomerase α
- Glycerophosphate Dehydrogenase Platinum Electrode
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry, medical
- Clinical Biochemistry