Ampa receptors exist in tunable mobile and immobile synaptic fractions in vivo

Haiwen Chen, Richard H. Roth, Elena Lopez-Ortega, Han L. Tan, Richard L. Huganir

Research output: Contribution to journalReview articlepeer-review

Abstract

AMPA receptor (AMPAR) mobility within synapses has been extensively studied in vitro. However, whether similar mobility properties apply to AMPARs in vivo has yet to be determined. Here, we use two-photon fluo-rescence recovery after photobleaching (FRAP) to study AMPAR mobility within individual dendritic spines in live animals using an overexpression vector. We demonstrate the existence of mobile and immobile fractions of AMPARs across multiple cortical regions and layers. Additionally, we find that AMPAR mobility can be al-tered in vivo in response to administration of corticosterone, a condition that mimics exposure to stress.

Original languageEnglish (US)
Article numberENEURO.0015-21.2021
JournaleNeuro
Volume8
Issue number3
DOIs
StatePublished - 2021

Keywords

  • GluA1
  • Plasticity
  • Stress
  • Synapses

ASJC Scopus subject areas

  • Neuroscience(all)

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