Ampa receptors exist in tunable mobile and immobile synaptic fractions in vivo

Haiwen Chen; Richard H. Roth; Elena Lopez-Ortega; Han L. Tan; Richard L. Huganir

doi:10.1523/ENEURO.0015-21.2021

Ampa receptors exist in tunable mobile and immobile synaptic fractions in vivo

Haiwen Chen, Richard H. Roth, Elena Lopez-Ortega, Han L. Tan, Richard L. Huganir

School of Medicine

Research output: Contribution to journal › Review article › peer-review

Abstract

AMPA receptor (AMPAR) mobility within synapses has been extensively studied in vitro. However, whether similar mobility properties apply to AMPARs in vivo has yet to be determined. Here, we use two-photon fluo-rescence recovery after photobleaching (FRAP) to study AMPAR mobility within individual dendritic spines in live animals using an overexpression vector. We demonstrate the existence of mobile and immobile fractions of AMPARs across multiple cortical regions and layers. Additionally, we find that AMPAR mobility can be al-tered in vivo in response to administration of corticosterone, a condition that mimics exposure to stress.

Original language	English (US)
Article number	ENEURO.0015-21.2021
Journal	eNeuro
Volume	8
Issue number	3
DOIs	https://doi.org/10.1523/ENEURO.0015-21.2021
State	Published - 2021

Keywords

GluA1
Plasticity
Stress
Synapses

ASJC Scopus subject areas

General Neuroscience

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10.1523/ENEURO.0015-21.2021

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title = "Ampa receptors exist in tunable mobile and immobile synaptic fractions in vivo",

abstract = "AMPA receptor (AMPAR) mobility within synapses has been extensively studied in vitro. However, whether similar mobility properties apply to AMPARs in vivo has yet to be determined. Here, we use two-photon fluo-rescence recovery after photobleaching (FRAP) to study AMPAR mobility within individual dendritic spines in live animals using an overexpression vector. We demonstrate the existence of mobile and immobile fractions of AMPARs across multiple cortical regions and layers. Additionally, we find that AMPAR mobility can be al-tered in vivo in response to administration of corticosterone, a condition that mimics exposure to stress.",

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doi = "10.1523/ENEURO.0015-21.2021",

language = "English (US)",

volume = "8",

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T1 - Ampa receptors exist in tunable mobile and immobile synaptic fractions in vivo

AU - Chen, Haiwen

AU - Roth, Richard H.

AU - Lopez-Ortega, Elena

AU - Tan, Han L.

AU - Huganir, Richard L.

PY - 2021

Y1 - 2021

N2 - AMPA receptor (AMPAR) mobility within synapses has been extensively studied in vitro. However, whether similar mobility properties apply to AMPARs in vivo has yet to be determined. Here, we use two-photon fluo-rescence recovery after photobleaching (FRAP) to study AMPAR mobility within individual dendritic spines in live animals using an overexpression vector. We demonstrate the existence of mobile and immobile fractions of AMPARs across multiple cortical regions and layers. Additionally, we find that AMPAR mobility can be al-tered in vivo in response to administration of corticosterone, a condition that mimics exposure to stress.

AB - AMPA receptor (AMPAR) mobility within synapses has been extensively studied in vitro. However, whether similar mobility properties apply to AMPARs in vivo has yet to be determined. Here, we use two-photon fluo-rescence recovery after photobleaching (FRAP) to study AMPAR mobility within individual dendritic spines in live animals using an overexpression vector. We demonstrate the existence of mobile and immobile fractions of AMPARs across multiple cortical regions and layers. Additionally, we find that AMPAR mobility can be al-tered in vivo in response to administration of corticosterone, a condition that mimics exposure to stress.

KW - GluA1

KW - Plasticity

KW - Stress

KW - Synapses

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U2 - 10.1523/ENEURO.0015-21.2021

DO - 10.1523/ENEURO.0015-21.2021

M3 - Review article

C2 - 33906969

AN - SCOPUS:85106337748

SN - 2373-2822

VL - 8

JO - eNeuro

JF - eNeuro

IS - 3

M1 - ENEURO.0015-21.2021

ER -

Ampa receptors exist in tunable mobile and immobile synaptic fractions in vivo

Abstract

Keywords

ASJC Scopus subject areas

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