AMPA receptor is involved in transmission of cone signal to ON bipolar cells in carp retina

Hai Huang, Dong Gen Luo, Yin Shen, Ai Jun Zhang, Ru Yang, Xiong Li Yang

Research output: Contribution to journalArticle

Abstract

The present work focuses on characterization of glutamate receptor subtypes mediating cone signal transmission to ON bipolar cells (BCs) in the carp retina, using intracellular recording techniques. Glutamate (5 mM) hyperpolarized cone-dominant ON BCs, which was associated with a suppression of light responses, whereas Co2+ (1 mM) depolarized these cells and suppressed their light responses. On the other hand, α-amino-3-hydroxy-5- methyl-4-isoxazolepropionic acid (AMPA) of 20 μM caused a membrane depolarization and blocked depolarizing light responses, L-2-amino-4- phosphonobutryic acid (L-AP4) was without effect. The effects of AMPA were reversed by coapplication of GYKI 52466, an AMPA receptor selective non-competitive antagonist, but persisted in the presence of picrotoxin and strychnine. For rod-dominant ON BCs, both L-AP4 and AMPA reversibly blocked depolarizing light responses, but with membrane potential changes of opposite polarities (hyperpolarization for L-AP4 and depolarization for AMPA). In the inner retina, AMPA depolarized transient ON-OFF amacrine cells and blocked both ON and OFF cone-driven depolarizing responses, but L-AP4 did not. These results suggest that AMPA receptors, but not L-AP4 receptors, are involved in synaptic transmission of cone signal to ON bipolar cells in carp retina.

Original languageEnglish (US)
Pages (from-to)86-93
Number of pages8
JournalBrain research
Volume1002
Issue number1-2
DOIs
StatePublished - Mar 26 2004

Keywords

  • AMPA receptor
  • Bipolar cell
  • Carp retina
  • ON signal pathway

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'AMPA receptor is involved in transmission of cone signal to ON bipolar cells in carp retina'. Together they form a unique fingerprint.

  • Cite this

    Huang, H., Luo, D. G., Shen, Y., Zhang, A. J., Yang, R., & Yang, X. L. (2004). AMPA receptor is involved in transmission of cone signal to ON bipolar cells in carp retina. Brain research, 1002(1-2), 86-93. https://doi.org/10.1016/j.brainres.2003.12.019