TY - JOUR
T1 - Ammonium sulfate and MALDI in-source decay
T2 - A winning combination for sequencing peptides
AU - Delvolve, Alice
AU - Woods, Amina S.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - In previous papers, we highlighted the role of ammonium sulfate in increasing peptide fragmentation by in-source decay ( ISD). The current work systematically investigated effects of matrix assisted laser desorption ionization (MALDI) extraction delay, peptide amino acid composition, matrix, and ammonium sulfate concentration on peptide ISD fragmentation. The data confirmed that ammonium sulfate increased peptides signal-to-noise ratio as well as their in-source fragmentation, resulting in complete sequence coverage regardless of the amino acid composition. This method is easy, inexpensive, and generates the peptides sequence instantly.
AB - In previous papers, we highlighted the role of ammonium sulfate in increasing peptide fragmentation by in-source decay ( ISD). The current work systematically investigated effects of matrix assisted laser desorption ionization (MALDI) extraction delay, peptide amino acid composition, matrix, and ammonium sulfate concentration on peptide ISD fragmentation. The data confirmed that ammonium sulfate increased peptides signal-to-noise ratio as well as their in-source fragmentation, resulting in complete sequence coverage regardless of the amino acid composition. This method is easy, inexpensive, and generates the peptides sequence instantly.
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U2 - 10.1021/ac901518a
DO - 10.1021/ac901518a
M3 - Article
C2 - 19877641
AN - SCOPUS:73249149948
SN - 0003-2700
VL - 81
SP - 9585
EP - 9589
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 23
ER -