Ammonium sulfate and MALDI in-source decay: A winning combination for sequencing peptides

Alice Delvolve, Amina S. Woods

Research output: Contribution to journalArticle

Abstract

In previous papers, we highlighted the role of ammonium sulfate in increasing peptide fragmentation by in-source decay ( ISD). The current work systematically investigated effects of matrix assisted laser desorption ionization (MALDI) extraction delay, peptide amino acid composition, matrix, and ammonium sulfate concentration on peptide ISD fragmentation. The data confirmed that ammonium sulfate increased peptides signal-to-noise ratio as well as their in-source fragmentation, resulting in complete sequence coverage regardless of the amino acid composition. This method is easy, inexpensive, and generates the peptides sequence instantly.

Original languageEnglish (US)
Pages (from-to)9585-9589
Number of pages5
JournalAnalytical Chemistry
Volume81
Issue number23
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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