Aminoglycoside levels in the rabbit vitreous cavity after intravenous administration

A. El-Massry; T. A. Meredith; H. E. Aguilar; A. Shaarawy; M. Kincaid; J. Dick; M. I.E. Mahmoud

doi:10.1016/S0002-9394(14)70487-8

Aminoglycoside levels in the rabbit vitreous cavity after intravenous administration

A. El-Massry, T. A. Meredith, H. E. Aguilar, A. Shaarawy, M. Kincaid, J. Dick, M. I.E. Mahmoud

School of Medicine

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

PURPOSE: To determine the penetration of gentamicin and amikacin into the rabbit vitreous cavity after their intravenous administration. METHODS: Gentamicin (1.6 mg/kg every 8 hours) and amikacin (6 mg/kg every 12 hours) were administered intravenously to 25 rabbits that had previously had the lens and vitreous removed from 43 eyes. For each drug, ocular inflammation was induced in one group of eyes by injection of heat-killed Staphylococcus epidermidis, while the other group was maintained as a control. Samples from the vitreous cavity were taken at regular intervals for 72 hours after beginning the intravenous medications and were analyzed for drug concentrations. RESULTS: The maximum intravitreal concentration ± SD achieved for gentamicin was 1.8 ± 0.5 μg/ml. The maximum intravitreal concentration for amikacin was 8.5 ± 3.2 μg/ml. Inflamed eyes demonstrated higher concentrations than did those without inflammation. CONCLUSIONS: In a rabbit model with conditions optimized to enhance penetration of antimicrobials into the vitreous cavity after intravenous administration, neither gentamicin nor amikacin penetrated sufficiently to reach potentially therapeutic concentrations consistently for either Pseudomonas or S epidermidis organisms.

Original language	English (US)
Pages (from-to)	684-689
Number of pages	6
Journal	American journal of ophthalmology
Volume	122
Issue number	5
DOIs	https://doi.org/10.1016/S0002-9394(14)70487-8
State	Published - 1996

ASJC Scopus subject areas

Ophthalmology

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@article{5a48cacd0c2b48fd9d2f3899f54ae136,

title = "Aminoglycoside levels in the rabbit vitreous cavity after intravenous administration",

abstract = "PURPOSE: To determine the penetration of gentamicin and amikacin into the rabbit vitreous cavity after their intravenous administration. METHODS: Gentamicin (1.6 mg/kg every 8 hours) and amikacin (6 mg/kg every 12 hours) were administered intravenously to 25 rabbits that had previously had the lens and vitreous removed from 43 eyes. For each drug, ocular inflammation was induced in one group of eyes by injection of heat-killed Staphylococcus epidermidis, while the other group was maintained as a control. Samples from the vitreous cavity were taken at regular intervals for 72 hours after beginning the intravenous medications and were analyzed for drug concentrations. RESULTS: The maximum intravitreal concentration ± SD achieved for gentamicin was 1.8 ± 0.5 μg/ml. The maximum intravitreal concentration for amikacin was 8.5 ± 3.2 μg/ml. Inflamed eyes demonstrated higher concentrations than did those without inflammation. CONCLUSIONS: In a rabbit model with conditions optimized to enhance penetration of antimicrobials into the vitreous cavity after intravenous administration, neither gentamicin nor amikacin penetrated sufficiently to reach potentially therapeutic concentrations consistently for either Pseudomonas or S epidermidis organisms.",

author = "A. El-Massry and Meredith, {T. A.} and Aguilar, {H. E.} and A. Shaarawy and M. Kincaid and J. Dick and Mahmoud, {M. I.E.}",

note = "Funding Information: From the Departments of Ophthalmology (Drs Meredith, Aguilar, Shaarawy, and Kincaid) and Pathology (Dr Kincaid), St Louis University School of Medicine, St Louis, Missouri; the Department of Microbiology, Johns Hopkins Hospital, Baltimore, Maryland (Dr Dick); and Johns Hopkins University School of Medicine, Baltimore, Maryland (Drs El-Massry and Mahmoud). This study was supported by N1H grant ROl EY-0574-0441 (Dr Meredith) and by an unrestricted grant from Research to Prevent Blindness, Ine, New York, New York.",

year = "1996",

doi = "10.1016/S0002-9394(14)70487-8",

language = "English (US)",

volume = "122",

pages = "684--689",

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TY - JOUR

T1 - Aminoglycoside levels in the rabbit vitreous cavity after intravenous administration

AU - El-Massry, A.

AU - Meredith, T. A.

AU - Aguilar, H. E.

AU - Shaarawy, A.

AU - Kincaid, M.

AU - Dick, J.

AU - Mahmoud, M. I.E.

N1 - Funding Information: From the Departments of Ophthalmology (Drs Meredith, Aguilar, Shaarawy, and Kincaid) and Pathology (Dr Kincaid), St Louis University School of Medicine, St Louis, Missouri; the Department of Microbiology, Johns Hopkins Hospital, Baltimore, Maryland (Dr Dick); and Johns Hopkins University School of Medicine, Baltimore, Maryland (Drs El-Massry and Mahmoud). This study was supported by N1H grant ROl EY-0574-0441 (Dr Meredith) and by an unrestricted grant from Research to Prevent Blindness, Ine, New York, New York.

PY - 1996

Y1 - 1996

N2 - PURPOSE: To determine the penetration of gentamicin and amikacin into the rabbit vitreous cavity after their intravenous administration. METHODS: Gentamicin (1.6 mg/kg every 8 hours) and amikacin (6 mg/kg every 12 hours) were administered intravenously to 25 rabbits that had previously had the lens and vitreous removed from 43 eyes. For each drug, ocular inflammation was induced in one group of eyes by injection of heat-killed Staphylococcus epidermidis, while the other group was maintained as a control. Samples from the vitreous cavity were taken at regular intervals for 72 hours after beginning the intravenous medications and were analyzed for drug concentrations. RESULTS: The maximum intravitreal concentration ± SD achieved for gentamicin was 1.8 ± 0.5 μg/ml. The maximum intravitreal concentration for amikacin was 8.5 ± 3.2 μg/ml. Inflamed eyes demonstrated higher concentrations than did those without inflammation. CONCLUSIONS: In a rabbit model with conditions optimized to enhance penetration of antimicrobials into the vitreous cavity after intravenous administration, neither gentamicin nor amikacin penetrated sufficiently to reach potentially therapeutic concentrations consistently for either Pseudomonas or S epidermidis organisms.

AB - PURPOSE: To determine the penetration of gentamicin and amikacin into the rabbit vitreous cavity after their intravenous administration. METHODS: Gentamicin (1.6 mg/kg every 8 hours) and amikacin (6 mg/kg every 12 hours) were administered intravenously to 25 rabbits that had previously had the lens and vitreous removed from 43 eyes. For each drug, ocular inflammation was induced in one group of eyes by injection of heat-killed Staphylococcus epidermidis, while the other group was maintained as a control. Samples from the vitreous cavity were taken at regular intervals for 72 hours after beginning the intravenous medications and were analyzed for drug concentrations. RESULTS: The maximum intravitreal concentration ± SD achieved for gentamicin was 1.8 ± 0.5 μg/ml. The maximum intravitreal concentration for amikacin was 8.5 ± 3.2 μg/ml. Inflamed eyes demonstrated higher concentrations than did those without inflammation. CONCLUSIONS: In a rabbit model with conditions optimized to enhance penetration of antimicrobials into the vitreous cavity after intravenous administration, neither gentamicin nor amikacin penetrated sufficiently to reach potentially therapeutic concentrations consistently for either Pseudomonas or S epidermidis organisms.

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ER -

Aminoglycoside levels in the rabbit vitreous cavity after intravenous administration

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