Amino Acids Mediate Postprandial Jejunal Proabsorption

Oscar J. Hines, Anton J. Bilchik, Stanley W. Ashley, Edward E. Whang, Carson D. Liu, Michael J. Zinner, David W. McFadden

Research output: Contribution to journalArticle

Abstract

Ingestion of a meal stimulates small intestinal ion and water transport. Current evidence suggests that this response, termed proabsorption, is primarily mediated by the apical Na+/glucose cotransporter. Like glucose, the majority of amino acid absorption occurs by Na+-dependent, secondary active transport. The purpose of this study was to determine the role of amino acid transport in meal-induced jejunal ion and water absorption in vivo. Exteriorized, neurovascularly intact jejunal loops measuring 25 cm were created in six female mongrel dogs, and the dogs were allowed to recover for 2 weeks. After an overnight fast, the loops were perfused with a standard buffer containing 10 mM aspartate, leucine, glycine, or lysine. Net water and electrolyte absorption before and after a mixed meal was calculated using [14C]polyethylene glycol as a volume marker. Aspartic acid, leucine, glycine, and lysine are each transported by a separate transporter system. Except for lysine, each amino acid significantly (P <0.05) potentiated sodium and water absorption after a meal. In addition, this effect was at least as great as that seen with 10 mM glucose. These results demonstrate that amino acid transporter, like the Na+/glucose cotransporter, mediates meal-induced jejunal sodium and water absorption and may be as important in the proabsorptive response.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalJournal of Surgical Research
Volume58
Issue number1
DOIs
StatePublished - Jan 1995
Externally publishedYes

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Meals
Amino Acids
Water
Lysine
Glucose
Aspartic Acid
Leucine
Glycine
Sodium
Dogs
Amino Acid Transport Systems
Active Biological Transport
Ion Transport
Electrolytes
Buffers
Eating
Ions

ASJC Scopus subject areas

  • Surgery

Cite this

Hines, O. J., Bilchik, A. J., Ashley, S. W., Whang, E. E., Liu, C. D., Zinner, M. J., & McFadden, D. W. (1995). Amino Acids Mediate Postprandial Jejunal Proabsorption. Journal of Surgical Research, 58(1), 81-85. https://doi.org/10.1006/jsre.1995.1013

Amino Acids Mediate Postprandial Jejunal Proabsorption. / Hines, Oscar J.; Bilchik, Anton J.; Ashley, Stanley W.; Whang, Edward E.; Liu, Carson D.; Zinner, Michael J.; McFadden, David W.

In: Journal of Surgical Research, Vol. 58, No. 1, 01.1995, p. 81-85.

Research output: Contribution to journalArticle

Hines, OJ, Bilchik, AJ, Ashley, SW, Whang, EE, Liu, CD, Zinner, MJ & McFadden, DW 1995, 'Amino Acids Mediate Postprandial Jejunal Proabsorption', Journal of Surgical Research, vol. 58, no. 1, pp. 81-85. https://doi.org/10.1006/jsre.1995.1013
Hines OJ, Bilchik AJ, Ashley SW, Whang EE, Liu CD, Zinner MJ et al. Amino Acids Mediate Postprandial Jejunal Proabsorption. Journal of Surgical Research. 1995 Jan;58(1):81-85. https://doi.org/10.1006/jsre.1995.1013
Hines, Oscar J. ; Bilchik, Anton J. ; Ashley, Stanley W. ; Whang, Edward E. ; Liu, Carson D. ; Zinner, Michael J. ; McFadden, David W. / Amino Acids Mediate Postprandial Jejunal Proabsorption. In: Journal of Surgical Research. 1995 ; Vol. 58, No. 1. pp. 81-85.
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