Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability

Sarah M. Short, Emmanuel F. Mongodin, Hannah J. MacLeod, Octavio A.C. Talyuli, George Dimopoulos

Research output: Contribution to journalArticle

Abstract

The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmission. In this work, we report significant variation in female adult midgut bacterial load between strains of A. aegypti which vary in their susceptibility to dengue virus. Composition of the midgut microbiome was similar overall between the strains, with 81–92% of reads coming from the same five bacterial families, though we did detect differences in the presence of some bacterial families including Flavobacteriaceae and Entobacteriaceae. We conducted transcriptomic analysis on the two mosquito strains that showed the greatest difference in bacterial load, and found that they differ in transcript abundance of many genes implicated in amino acid metabolism, in particular the branched chain amino acid degradation pathway. We then silenced this pathway by targeting multiple genes using RNA interference, which resulted in strain-specific bacterial proliferation, thereby eliminating the difference in midgut bacterial load between the strains. This suggests that the branched chain amino acid (BCAA) degradation pathway controls midgut bacterial load, though the mechanism underlying this remains unclear. Overall, our results indicate that amino acid metabolism can act to influence the midgut microbiota. Moreover, they suggest that genetic or physiological variation in BCAA degradation pathway activity may in part explain midgut microbiota variation in the field.

Original languageEnglish (US)
Article numbere0005677
JournalPLoS Neglected Tropical Diseases
Volume11
Issue number7
DOIs
StatePublished - Jul 28 2017

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Aedes
Microbiota
Amino Acids
Bacterial Load
Branched Chain Amino Acids
Culicidae
Flavobacteriaceae
Dengue Virus
Infectious Disease Transmission
Gene Targeting
RNA Interference
Genes

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability. / Short, Sarah M.; Mongodin, Emmanuel F.; MacLeod, Hannah J.; Talyuli, Octavio A.C.; Dimopoulos, George.

In: PLoS Neglected Tropical Diseases, Vol. 11, No. 7, e0005677, 28.07.2017.

Research output: Contribution to journalArticle

Short, Sarah M.; Mongodin, Emmanuel F.; MacLeod, Hannah J.; Talyuli, Octavio A.C.; Dimopoulos, George / Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability.

In: PLoS Neglected Tropical Diseases, Vol. 11, No. 7, e0005677, 28.07.2017.

Research output: Contribution to journalArticle

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