Amiloride inhibits meal-stimulated colonic absorption

Edward E. Whang, James C. Dunn, Carson D. Liu, Todd Newton, Michael J. Zinner, David W. Mcfadden, S. W. Ashley

Research output: Contribution to journalArticle

Abstract

Meal-stimulated colonic absorption has recently been described, but the cellular transport mechanisms mediating this response are unknown. The purpose of this study was to determine the contribution of Na+ transport pathways to colonic proabsorption. Distal colonic Thiry-Vella loops were constructed in six dogs. Absorption was measured by infusing the loops with a physiological electrolyte solution containing [14C]polyethylene glycol as the impermeant marker. In the first set of experiments, the dose dependence of amiloride-induced inhibition of basal colonic absorption was determined. In the second set of experiments the effect of amiloride, which inhibits both Na+ channels and Na+/H+ exchange in colonocytes, on meal-stimulated colonic absorption was determined. Luminal amiloride inhibited basal colonic absorption in a dose-dependent manner, with significant reductions in Na+ absorption occurring with concentrations of 10-2 M and higher. Infusion with 10-3 M amiloride, a concentration that did not alter basal absorption, resulted in significant reductions in postprandial water, Na+, and Cl absorption. These results suggest that meal-stimulated colonic absorption is mediated, at least in part, by transcellular Na+ absorptive pathways.

Original languageEnglish (US)
Pages (from-to)303-306
Number of pages4
JournalJournal of Surgical Research
Volume60
Issue number2
DOIs
StatePublished - Feb 1 1996

ASJC Scopus subject areas

  • Surgery

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    Whang, E. E., Dunn, J. C., Liu, C. D., Newton, T., Zinner, M. J., Mcfadden, D. W., & Ashley, S. W. (1996). Amiloride inhibits meal-stimulated colonic absorption. Journal of Surgical Research, 60(2), 303-306. https://doi.org/10.1006/jsre.1996.0047