Amide proton transfer imaging of 9L gliosarcoma and human glioblastoma xenografts

Amandeep Salhotra, Bachchu Lal, John Laterra, Phillip Zhe Sun, Peter C.M. van Zijl, Jinyuan Zhou

Research output: Contribution to journalArticle

Abstract

Amide proton transfer (APT) imaging is a variant of magnetization transfer (MT) imaging, in which the contrast is determined by a change in water intensity due to chemical exchange with saturated amide protons of endogenous mobile proteins and peptides. In this study, eight Fisher 344 rats implanted with 9L gliosarcoma cells and six nude rats implanted with human glioblastoma cells were imaged at 4.7 T. There were increased signal intensities in tumors in the APT-weighted images. The contrast of APT imaging between the tumor and contralateral brain tissue was about 3.9% in water intensity (1.49 ± 0.66% vs -2.36 ± 0.19%) for the more uniformly hypercellular 9L brain tumors, and it was reduced to 1.6% (-1.18 ± 0.60% vs -2.77 ± 0.42%) for the human glioblastoma xenografts that contained hypocellular zones of necrosis. The preliminary results show that the APT technique at the protein level may provide a unique MRI contrast for the characterization of brain tumors.

Original languageEnglish (US)
Pages (from-to)489-497
Number of pages9
JournalNMR in biomedicine
Volume21
Issue number5
DOIs
StatePublished - Jun 2008

Keywords

  • 9L gliosarcoma
  • APT imaging
  • Amide proton transfer (APT)
  • Brain tumor
  • Magnetization transfer
  • Protein

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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