TY - JOUR
T1 - Ameliorating Effects and Mechanisms of Intra-Operative Vagal Nerve Stimulation on Postoperative Recovery After Sleeve Gastrectomy in Rats
AU - Tian, Ming
AU - Alimujiang, Maisiyiti
AU - Chen, Jiande Dz
N1 - Publisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Purpose: Sleeve gastrectomy is a common bariatric procedure. The aim of this study was to explore the effects and mechanisms of intra-operative vagal nerve stimulation (iVNS) on postoperative recovery after sleeve gastrectomy in rats. Materials and Methods: Rats were randomly divided into groups of sham-iVNS (n = 12) and iVNS (n = 12), and received sleeve gastrectomy, and were implanted electrodes for the electrocardiogram (ECG) and gastric pace-making activity or slow wave (GSW). iVNS or sham-iVNS was performed during surgery. Postoperative animal behaviors, pain, gastrointestinal functions, autonomic functions, inflammatory cytokines, and hormones in plasma were assessed. Results: In comparison with sham-iVNS, (1) iVNS accelerated the first drinking time (P = 0.01) and first defecation time (P = 0.02), increased the weight of stool in postoperative day (POD) 1 (P = 0.01) and POD3 (P = 0.01), and reduced postoperative pain at 24 h after surgery (P = 0.01); (2) physiologically, iVNS improved gastric emptying (P < 0.01) at 72 h after surgery, and increased the dominant frequency (P < 0.01) and the percentage of normal GSW (P = 0.04) at 6 h after surgery; (3) mechanistically, iVNS increased vagal activity and decreased sympathovagal ratio at 6 h (vagal, P < 0.01; sympathovagal ratio, P < 0.01) and 24 h (vagal, P = 0.02; sympathovagal ratio, P = 0.01) after surgery, increased the plasma pancreatic polypeptide (P = 0.03) at 1 h after surgery, and decreased the plasma tumor necrosis factor-α (TNF-α) at 1 h (P = 0.02) and 6 h (P = 0.03) and the plasma interleukin-6 (IL-6) at 1 h (P = 0.02) after surgery. Conclusion: Intra-operative VNS is effective in accelerating post-surgical recovery in rats after sleeve gastrectomy by enhancing gastric pace-making activity and suppressing proinflammatory cytokines mediated via the autonomic mechanisms.
AB - Purpose: Sleeve gastrectomy is a common bariatric procedure. The aim of this study was to explore the effects and mechanisms of intra-operative vagal nerve stimulation (iVNS) on postoperative recovery after sleeve gastrectomy in rats. Materials and Methods: Rats were randomly divided into groups of sham-iVNS (n = 12) and iVNS (n = 12), and received sleeve gastrectomy, and were implanted electrodes for the electrocardiogram (ECG) and gastric pace-making activity or slow wave (GSW). iVNS or sham-iVNS was performed during surgery. Postoperative animal behaviors, pain, gastrointestinal functions, autonomic functions, inflammatory cytokines, and hormones in plasma were assessed. Results: In comparison with sham-iVNS, (1) iVNS accelerated the first drinking time (P = 0.01) and first defecation time (P = 0.02), increased the weight of stool in postoperative day (POD) 1 (P = 0.01) and POD3 (P = 0.01), and reduced postoperative pain at 24 h after surgery (P = 0.01); (2) physiologically, iVNS improved gastric emptying (P < 0.01) at 72 h after surgery, and increased the dominant frequency (P < 0.01) and the percentage of normal GSW (P = 0.04) at 6 h after surgery; (3) mechanistically, iVNS increased vagal activity and decreased sympathovagal ratio at 6 h (vagal, P < 0.01; sympathovagal ratio, P < 0.01) and 24 h (vagal, P = 0.02; sympathovagal ratio, P = 0.01) after surgery, increased the plasma pancreatic polypeptide (P = 0.03) at 1 h after surgery, and decreased the plasma tumor necrosis factor-α (TNF-α) at 1 h (P = 0.02) and 6 h (P = 0.03) and the plasma interleukin-6 (IL-6) at 1 h (P = 0.02) after surgery. Conclusion: Intra-operative VNS is effective in accelerating post-surgical recovery in rats after sleeve gastrectomy by enhancing gastric pace-making activity and suppressing proinflammatory cytokines mediated via the autonomic mechanisms.
KW - Inflammation
KW - Intra-operative vagal nerve stimulation
KW - Motility
KW - Pain
KW - Postoperative recovery
KW - Sleeve gastrectomy
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U2 - 10.1007/s11695-020-04626-w
DO - 10.1007/s11695-020-04626-w
M3 - Article
C2 - 32318995
AN - SCOPUS:85083703754
VL - 30
SP - 2980
EP - 2987
JO - Obesity Surgery
JF - Obesity Surgery
SN - 0960-8923
IS - 8
ER -